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Primer Extension Reaction at the Gold Electrode Surface
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SYSNO ASEP 0506714 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Primer Extension Reaction at the Gold Electrode Surface Author(s) Vidláková, Pavlína (BFU-R)
Havran, Luděk (BFU-R) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 3 Source Title XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference. - Ústí nad Labem : Srsenová Lenka - Best Servis, 2015 / Navrátil Tomáš ; Fojta M. ; Schwarzová Karolína - ISBN 978-80-905221-3-8 Pages č. 2015 (2015), s. 269-271 Number of pages 3 s. Publication form Print - P Action Moderní elektrochemické metody /35./ Event date 18.05.2015 - 22.05.2015 VEvent location Jetřichovice Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords dna ; immobilization ; optimization Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects GAP206/11/1638 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000358792900060 Annotation One option of covalent DNA labeling is incorporation of chemically modified nucleotides using corresponding deoxynucleotide triphosphates into DNA molecules using polymerase-catalyzed primer extension. Primer extension is usually performed in solution and then the product is analyzed, often after a suitable separation. In our study we tested the possibility to prepare oligonucleotides with enzymatically incorporated anthraquinone labeled nucleotides using primer extension on templates immobilize at a gold electrode surface. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020
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