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Constant Current Chronopotentiometry as a Method for Detection of Singlet Oxygen Protein Damage
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SYSNO ASEP 0506684 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Constant Current Chronopotentiometry as a Method for Detection of Singlet Oxygen Protein Damage Author(s) Ostatná, Veronika (BFU-R) RID, ORCID
Vargová, Veronika (BFU-R)
Černocká, Hana (BFU-R) RID, ORCID
Paleček, Emil (BFU-R) RID, ORCIDNumber of authors 4 Source Title XXXVI. Moderní elektrochemické metody. Sborník přednášek. - Ústí nad Labem : Best servis, 2016 / Navrátil T. ; Fojta M. ; Schwarzová K. - ISBN 978-80-905221-4-5 Pages č. 2016 (2016), s. 158-160 Number of pages 3 s. Publication form Print - P Action Moderní elektrochemické metody /36./ Event date 23.05.2016 - 27.05.2016 VEvent location Jetřichovice Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords carbon electrodes ; affinity tags ; mercury ; purification Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects GA13-00956S GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000389898200037 Annotation Proteins are one of the major targets for oxidative damage in the cell.Indirect non-radical oxidation of the protein via formation and subsequent reaction with single oxygen (O-1(2))is one of the major processes.In this work we studied the single oxygen(O-1(2))-mediated oxidation of bovine serum albumin (BSA) by constant current chronopotentiometry in combination with mercury electrode.Our chronopotentiometric data show that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Our method utilizing intrinsic electrochemical signal of proteins provides a sensitive detection methods for minor damages in various proteins. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020
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