Biomimetic modification of dual porosity poly(2-hydroxyethyl methacrylate) hydrogel scaffolds - porosity and stem cell growth evaluation

0506355 - ÚMCH 2020 RIV GB eng J - Journal Article
Janoušková, Olga - Přádný, Martin - Vetrík, Miroslav - Chylíková Krumbholcová, Eva - Michálek, Jiří - Dušková-Smrčková, Miroslava
Biomimetic modification of dual porosity poly(2-hydroxyethyl methacrylate) hydrogel scaffolds - porosity and stem cell growth evaluation.
Biomedical Materials. Roč. 14, č. 5 (2019), s. 1-13, č. článku 055004. ISSN 1748-6041. E-ISSN 1748-605X
R&D Projects: GA ČR(CZ) GA17-08531S; GA ČR(CZ) GA17-11140S
Institutional support: RVO:61389013
Keywords : hydrogel * poly(2-hydroxyethyl methacrylate) * embryonic stem cells
OECD category: Polymer science
Impact factor: 3.174, year: 2019
Method of publishing: Limited access
https://iopscience.iop.org/article/10.1088/1748-605X/ab2856

The macroporous synthetic poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels as 3D cellular scaffolds with specific internal morphology, so called dual pore size, were designed and studied. The morphological microstructure of hydrogels was characterized in the gel swollen state and the susceptibility of gels for stem cells was evaluated. The effect of specific chemical groups covalently bound in the hydrogel network by copolymerization on cell adhesion and growth, followed by effect of laminin coating were investigated. The evaluated gels contained either carboxyl groups of the methacrylic acid or quaternary ammonium groups brought by polymerizable ammonium salt or their combinations. The morphology of swollen gel was visualized using the laser scanning confocal microscopy. All hydrogels had very similar porous structures – their matrices contained large pores (up to 102 μm) surrounded with gel walls with small pores (100 μm). The total pore volume in hydrogels swollen in buffer solution ranged between 69 and 86 vol%. Prior to the seeding of the mouse embryonal stem cells, the gels were coated with laminin. The hydrogel with quaternary ammonium groups (with or without laminin) stimulated the cell growth the most. The laminin coating lead to a significant and quaternary ammonium groups. The gel chemical modification influenced also the topology of cell coverage that ranged from individual cell clusters to well dispersed multi cellular structures. Findings in this study point out the laser scanning confocal microscopy as an irreplaceable method for a precise and quick assessment of the hydrogel morphology. In addition, these findings help to optimize the chemical composition of the hydrogel scaffold through the combination of chemical and biological factors leading to intensive cell attachment and proliferation.
Permanent Link: http://hdl.handle.net/11104/0298184