Molecular cytogenetic characterisation of Elytrigia ×mucronata, a natural hybrid of E. intermedia and E. repens (Triticeae, Poaceae)

0505859 - BÚ 2020 RIV GB eng J - Journal Article
Paštová, Ladislava - Belyayev, Alexander - Mahelka, Václav
Molecular cytogenetic characterisation of Elytrigia ×mucronata, a natural hybrid of E. intermedia and E. repens (Triticeae, Poaceae).
BMC Plant Biology. Roč. 19, 31 May (2019), s. 1-15, č. článku 230. ISSN 1471-2229. E-ISSN 1471-2229
R&D Projects: GA ČR GA17-06548S
Institutional support: RVO:67985939
Keywords : allopolyploids * Elytrigia * GISH
OECD category: Plant sciences, botany
Impact factor: 3.497, year: 2019
Method of publishing: Open access

There are presented data from the molecular cytogenetic analysis of three cytotypes of Elytrigia ×mucronata using sequential fluorescence (5S rDNA, 18S rDNA and pSc119.2 probes) and genomic in situ hybridisation (four genomic probes of diploid taxa, i.e., Aegilops, Dasypyrum, Hordeum and Pseudoroegneria).The following different genomic constitutions were observed for E. ×mucronata. Hexaploid plants exhibited three chromosome sets from Pseudoroegneria and one chromosome set each from Aegilops, Hordeum and Dasypyrum. Heptaploid plants harboured the six chromosome sets of the hexaploid plants and an additional Pseudoroegneria chromosome set. Nonaploid cytotypes differed in their genomic constitutions, reflecting different origins through the fusion of reduced and unreduced gametes. The hybridisation patterns of repetitive sequences (5S rDNA, 18S rDNA, and pSc119.2) in E. ×mucronata varied between and within cytotypes. Chromosome alterations that were not identified in the parental species were found in both heptaploid and some nonaploid plants.
Permanent Link: http://hdl.handle.net/11104/0297227