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The Low Temperature Method for Environmental Scanning Electron Microscopy – a New Method for Observation of Diatom Assemblages in vivo
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SYSNO ASEP 0504760 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Low Temperature Method for Environmental Scanning Electron Microscopy – a New Method for Observation of Diatom Assemblages in vivo Author(s) Fránková, Markéta (BU-J) RID, ORCID
Poulíčková, A. (CZ)
Neděla, Vilém (UPT-D) RID, ORCID, SAI
Tihlaříková, Eva (UPT-D) RID, ORCID, SAI
Šumberová, Kateřina (BU-J) RID, ORCID
Letáková, M. (CZ)Source Title Diatom Research - ISSN 0269-249X
Roč. 33, č. 3 (2018), s. 397-403Number of pages 7 s. Language eng - English Country GB - United Kingdom Keywords living diatoms ; epiphyton ; low temperature method for ESEM Subject RIV EH - Ecology, Behaviour OECD category Plant sciences, botany Subject RIV - cooperation Institute of Scientific Instruments - Optics, Masers, Lasers R&D Projects LD14045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support BU-J - RVO:67985939 ; UPT-D - RVO:68081731 UT WOS 000459800300008 EID SCOPUS 85059693525 DOI 10.1080/0269249X.2018.1545703 Annotation The Low Temperature Method for sample stabilization in environmental scanning electron microscopy (LTM for ESEM) is a promising tool for the observation of diatom assemblages in vivo. Use of the environmental scanning electron microscope, in comparison to the conventional scanning electron microscope, enables study of fresh material without any chemical pretreatment and conductive coating. The newly developed Low Temperature Method, introduced in this paper, offers higher resolution and better resistance of wet samples to radiation damage. We used natural epiphytic algal assemblages to image 3D structure of: i) biofilm/periphyton and its physical complexity, ii) diatoms with their extracellular mucilaginous secretions enabling cells to attach to the substrate, iii) diatom colony formation, and iv) intact diatom cells in contrast to separated empty valves observed in the conventional scanning electron microscope. This study demonstrates the potential of this new method for environmental scanning electron microscopy in diatom biology and ecology in comparison with other imaging methods. Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2020 Electronic address http://hdl.handle.net/11104/0296319
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