Number of the records: 1
Modification of Calcium Retention Capacity of Rat Liver Mitochondria by Phosphate and Tert-Butyl Hydroperoxide
- 1.
SYSNO ASEP 0504504 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Modification of Calcium Retention Capacity of Rat Liver Mitochondria by Phosphate and Tert-Butyl Hydroperoxide Author(s) Endlicher, R. (CZ)
Drahota, Zdeněk (FGU-C) RID, ORCID
Červinková, Z. (CZ)Source Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 68, č. 1 (2019), s. 59-65Number of pages 7 s. Language eng - English Country CZ - Czech Republic Keywords liver mitochondria ; calcium retention capacity ; mitochondrial permeability transition pore ; phosphate ; tert-butyl hydroperoxide Subject RIV ED - Physiology OECD category Physiology (including cytology) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000460617400006 EID SCOPUS 85062586692 DOI 10.33549/physiolres.933912 Annotation By determining the calcium retention capacity (CRC) of rat liver mitochondria, we confirmed and extended previous observations describing the activation of mitochondrial swelling by phosphate and tert-butyl hydroperoxide (t-BHP). Using CRC measurements, we showed that both phosphate and t-BHP decrease the extent of calcium accumulation required for the full mitochondrial permeability transition pore (MPTP) opening to 35 % of control values and to only 15 % when both phosphate and t-BHP are present in the medium. When changes in fluorescence were evaluated at higher resolution, we observed that in the presence of cyclosporine A fluorescence values return after each Ca2+ addition to basal values obtained before the Ca2+ addition. This indicates that the MPTP remains closed. However, in the absence of cyclosporine A, the basal fluorescence after each Ca2+ addition continuously increased. This increase was potentiated both by phosphate and t-BHP until the moment when the concentration of intramitochondrial calcium required for the full opening of the MPTP was reached. We conclude that in the absence of cyclosporine A, the MPTP is slowly opened after each Ca2+ addition and that this rate of opening can be modified by various factors such as the composition of the media and the experimental protocol used. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2020 Electronic address https://www.biomed.cas.cz/physiolres/pdf/2019/68_59.pdf
Number of the records: 1