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Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis
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SYSNO ASEP 0504121 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis Author(s) Skalák, J. (CZ)
Vercruyssen, L. (BE)
Claeys, H. (BE)
Hradilová, J. (CZ)
Černý, M. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Plačková, Lenka (UEB-Q) ORCID, RID
Saiz-Fernández, I. (CZ)
Skaláková, P. (CZ)
Coppens, F. (CZ)
D'hondt, B. (BE)
Koukalová, Š. (CZ)
Zouhar, J. (CZ)
Inzé, D. (BE)
Brzobohatý, Břetislav (BFU-R) RID, ORCIDNumber of authors 15 Source Title Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 97, č. 5 (2019), s. 805-824Number of pages 20 s. Language eng - English Country GB - United Kingdom Keywords Arabidopsis thaliana ; cytokinin ; leaf development ; proteome ; transcriptome Subject RIV ED - Physiology OECD category Biochemical research methods Subject RIV - cooperation Institute of Biophysics - Botanics R&D Projects GA17-04607S GA ČR - Czech Science Foundation (CSF) GA18-07563S GA ČR - Czech Science Foundation (CSF) LQ1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 ; BFU-R - RVO:68081707 UT WOS 000462684900002 EID SCOPUS 85062641549 DOI 10.1111/tpj.14285 Annotation The phytohormone cytokinin has been shown to affect many aspects of plant development ranging from the regulation of the shoot apical meristem to leaf senescence. However, some studies have reported contradictory effects of cytokinin on leaf physiology. Therefore cytokinin treatments cause both chlorosis and increased greening and both lead to decrease or increase in cell size. To elucidate this multifaceted role of cytokinin in leaf development, we have employed a system of temporal controls over the cytokinin pool and investigated the consequences of modulated cytokinin levels in the third leaf of Arabidopsis. We show that, at the cell proliferation phase, cytokinin is needed to maintain cell proliferation by blocking the transition to cell expansion and the onset of photosynthesis. Transcriptome profiling revealed regulation by cytokinin of a gene suite previously shown to affect cell proliferation and expansion and thereby a molecular mechanism by which cytokinin modulates a molecular network underlying the cellular responses. During the cell expansion phase, cytokinin stimulates cell expansion and differentiation. Consequently, a cytokinin excess at the cell expansion phase results in an increased leaf and rosette size fueled by higher cell expansion rate, yielding higher shoot biomass. Proteome profiling revealed the stimulation of primary metabolism by cytokinin, in line with an increased sugar content that is expected to increase turgor pressure, representing the driving force of cell expansion. Therefore, the developmental timing of cytokinin content fluctuations, together with a tight control of primary metabolism, is a key factor mediating transitions from cell proliferation to cell expansion in leaves. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020 Electronic address http://doi.org/10.1111/tpj.14285
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