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Cytological, Biochemical and Molecular Events of the Embryogenic State in Douglas-fir (Pseudotsuga menziesii [Mirb.])
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SYSNO ASEP 0502551 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cytological, Biochemical and Molecular Events of the Embryogenic State in Douglas-fir (Pseudotsuga menziesii [Mirb.]) Author(s) Gautier, F. (FR)
Label, P. (FR)
Eliášová, Kateřina (UEB-Q) RID, ORCID
Leple, J.C. (FR)
Motyka, Václav (UEB-Q) RID, ORCID
Boizot, N. (FR)
Vondráková, Zuzana (UEB-Q) RID, ORCID
Malbeck, Jiří (UEB-Q) RID, ORCID
Trávníčková, Alena (UEB-Q) ORCID
Le Metté, C. (FR)
Lesage-Descauses, M. C. (FR)
Lomenech, A. M. (FR)
Trontin, J.F. (FR)
Costa, G. (PT)
Lelu-Walter, M.A. (FR)
Teyssier, C. (FR)Number of authors 16 Article number 118 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 10, FEB 28 (2019)Number of pages 26 s. Language eng - English Country CH - Switzerland Keywords comparative proteomic analysis ; transcription factor family ; early somatic embryogenesis ; abscisic-acid ; nonembryogenic tissues ; sugar transporters ; genome sequence ; cell fate ; auxin ; expression ; embryonal mass ; non-embryogenic callus ; histo-cytology ; soluble carbohydrate ; phytohormone ; proteomic ; network ; transcriptomic Subject RIV EF - Botanics OECD category Biochemical research methods R&D Projects GA16-14649S GA ČR - Czech Science Foundation (CSF) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC17030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000459855700001 DOI 10.3389/fpls.2019.00118 Annotation Somatic embryogenesis techniques have been developed for most coniferous species, but only using very juvenile material. To extend the techniques' scope, better integrated understanding of the key biological, physiological and molecular characteristics of embryogenic state is required. Therefore, embryonal masses (EMs) and non-embryogenic calli (NECs) have been compared during proliferation at multiple levels. EMs and NECs originating from a single somatic embryo (isogenic lines) of each of three unrelated genotypes were used in the analyses, which included comparison of the lines' anatomy by transmission light microscopy, transcriptomes by RNAseq Illumina sequencing, proteomes by free-gel analysis, contents of endogenous phytohormones (indole-3-acetic acid, cytokinins and ABA) by LC-MS analysis, and soluble sugar contents by HPLC. EMs were characterized by upregulation (relative to levels in NECs) of transcripts, proteins, transcription factors and active cytokinins associated with cell differentiation accompanied by histological, carbohydrate content and genetic markers of cell division. In contrast, NECs were characterized by upregulation (relative to levels in EMs) of transcripts, proteins and products associated with responses to stimuli (ABA, degradation forms of cytokinins, phenols), oxidative stress (reactive oxygen species) and carbohydrate storage (starch). Sub-Network Enrichment Analyses that highlighted functions and interactions of transcripts and proteins that significantly differed between EMs and NECs corroborated these findings. The study shows the utility of a novel approach involving integrated multi-scale transcriptomic, proteomic, biochemical, histological and anatomical analyses to obtain insights into molecular events associated with embryogenesis and more specifically to the embryogenic state of cell in Douglas-fir. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2019
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