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Social defeat stimulates local glucocorticoid regeneration in lymphoid organs
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SYSNO ASEP 0498848 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Social defeat stimulates local glucocorticoid regeneration in lymphoid organs Author(s) Ergang, Peter (FGU-C) RID, ORCID
Mikulecká, Anna (FGU-C) RID, ORCID
Vodička, Martin (FGU-C) RID, ORCID, SAI
Vagnerová, Karla (FGU-C) RID, ORCID
Mikšík, Ivan (FGU-C) RID, ORCID, SAI
Pácha, Jiří (FGU-C) RID, ORCIDSource Title Endocrine Connections. - : BioScientifica - ISSN 2049-3614
Roč. 7, č. 12 (2018), s. 1389-1396Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords glucocorticoid metabolism ; lymhoid organs ; Lewis rats ; Fisher 344 rats ; social stress Subject RIV ED - Physiology OECD category Physiology (including cytology) R&D Projects GA15-07268S GA ČR - Czech Science Foundation (CSF) GA18-02993S GA ČR - Czech Science Foundation (CSF) Institutional support FGU-C - RVO:67985823 UT WOS 000456847800027 EID SCOPUS 85065087914 DOI 10.1530/EC-18-0319 Annotation Stress is an important risk factors for human diseases. It activates the hypothalamic-pituitary-adrenal (HPA) axis and increases plasma glucocorticoids, which are powerful regulators of immune system. The response of the target cells to glucocorticoids depends not only on the plasma concentrations of cortisol and corticosterone but also on their local metabolism. This metabolism is catalyzed by 1111-hydroxysteroid dehydrogenases type 1 and 2, which interconvert glucocorticoid hormones cortisol and corticosterone and their 11-oxo metabolites cortisone and 11-dehydrocorticosterone. The goal of this study was to determine whether stress modulates glucocorticoid metabolism within lymphoid organs - the structures where immune cells undergo development and activation. Using the resident-intruder paradigm, we studied the effect of social stress on glucocorticoid metabolism in primary and secondary lymphoid organs of Fisher 344 (F344) and Lewis (LEW) rats, which exhibit marked differences in their HPA axis response to social stressors and inflammation. We show that repeated social defeat increased the regeneration of corticosterone from 11-dehydrocorticosterone in the thymus, spleen and mesenteric lymphatic nodes (MLN). Compared with the F344 strain, LEW rats showed higher corticosterone regeneration in splenocytes of unstressed rats and in thymic and MLN mobile cells after stress but corticosterone regeneration in the stroma of all lymphoid organs was similar in both strains. Inactivation of corticosterone to 11-dehydrocorticosterone was found only in the stroma of lymphoid organs but not in mobile lymphoid cells and was not upregulated by stress. Together, our findings demonstrate the tissue- and strain-dependent regeneration of glucocorticoids following social stress. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2019
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