Number of the records: 1
Total synthesis of [N-15]-labelled C6-substituted purines from [N-15]-formamide-easy preparation of isotopically labelled cytokinins and derivatives
- 1.
SYSNO ASEP 0498271 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Total synthesis of [N-15]-labelled C6-substituted purines from [N-15]-formamide-easy preparation of isotopically labelled cytokinins and derivatives Author(s) Buček, J. (CZ)
Zatloukal, M. (CZ)
Havlíček, Libor (UEB-Q) RID, ORCID
Plíhalová, Lucie (UEB-Q) ORCID, RID
Pospíšil, T. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Doležal, Karel (UEB-Q) RID, ORCID
Strnad, Miroslav (UEB-Q) RID, ORCIDNumber of authors 8 Article number 181322 Source Title Royal Society Open Science. - : Royal Society Publishing - ISSN 2054-5703
Roč. 5, č. 11 (2018)Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords cell-division ; biological-activity ; aromatic cytokinins ; plant-tissues ; adenine ; zeatin ; identification ; diphosphate ; mechanism ; thaliana ; synthesis ; N-15-labelled ; purine ; cytokinin Subject RIV ED - Physiology OECD category Biochemical research methods R&D Projects GA16-04184S GA ČR - Czech Science Foundation (CSF) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000451777500038 DOI 10.1098/rsos.181322 Annotation Cytokinins (CKs) and their metabolites and derivatives are essential for cell division, plant growth regulation and development. They are typically found at minute concentrations in plant tissues containing very complicated biological matrices. Therefore, defined standards labelled with stable isotopes are required for precise metabolic profiling and quantification of CKs, as well as in vivo elucidation of CK biosynthesis in various plant species. In this work, 11 [N-15]-labelled C6-purine derivatives were prepared, among them 5 aromatic (4, 5, 6, 7, 8) and 3 isoprenoid (9, 10, 11) CKs. Compared to current methods, optimized syntheses of 6-amino-9H-[N-15(5)]-purine (adenine) and 6-chloro-9H-[N-15(4)]-purine (6-chloropurine) were performed to achieve more effective, selective and generally easier approaches. The chemical identity and purity of prepared compounds were confirmed by physico-chemical analyses (TLC, HRMS, HPLC-MS, H-1, C-13, N-15 NMR). The presented approach is applicable for the synthesis of any other desired [N-15(4) ]-labelled C6-substituted purine derivatives. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2019
Number of the records: 1