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TATAA courses – 1 day Sample preparation and quality control of nucleic acids\n\n
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SYSNO ASEP 0496246 Document Type U - Organizing Conference, Workshop, Exhibition R&D Document Type W - Uspořádání workshopu R&D Document Type Není vybrán druh dokumentu Title TATAA courses – 1 day Sample preparation and quality control of nucleic acids
Author(s) Kubista, Mikael (BTO-N) RID
Valihrach, Lukáš (BTO-N) RID, ORCID
Smolík, Ondřej (BTO-N)
Androvič, Peter (BTO-N)
Rohlová, Eva (BTO-N)
Švec, David (BTO-N) RIDEvent type W - Workshop Event date 18.04.2018 - 18.04.2018 VEvent location Vestec, BIOCEV Country CZ - Czech Republic Event type WRD Total number of participants 12 Number of foreign participants 9 Language eng - English Keywords qPCR ; quality control ; reverese transcription Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 Annotation One of the most important requirements to get good results from qPCR experiments is to have a template of good quality. In most cases this means having an efficient sample preparation. This course module is focused on extraction of RNA and DNA to be used as template in qPCR and reverse transcription reactions. It also includes the important quality control steps needed to asure reliable results. The course avers : Overview of nucleic acid extraction methods. How to properly determine the concentration of purified nucleic acids. Storage of biological samples and purified nucleic acids. Quality control of purified material. Integrity of purified RNA. How to test for the presence of inhibitors. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2019 Electronic address prague@tataa.com
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