CUP-SHAPED COTYLEDON1 (CUC1) and CUC2 regulate cytokinin homeostasis to determine ovule number in Arabidopsis

0495915 - ÚEB 2019 RIV GB eng J - Journal Article
Cucinotta, M. - Manrique, S. J. G. - Cuesta, C. - Benková, E. - Novák, Ondřej - Colombo, L.
CUP-SHAPED COTYLEDON1 (CUC1) and CUC2 regulate cytokinin homeostasis to determine ovule number in Arabidopsis.
Journal of Experimental Botany. Roč. 69, č. 21 (2018), s. 5169-5176. ISSN 0022-0957. E-ISSN 1460-2431
R&D Projects: GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : annotation data * gene ontology * carpel margin * thaliana * receptors * tissues * auxin * metabolism * pathways * family * Arabidopsis * cytokinin * hormones * ovule development * pistil development * transcriptional regulation
OECD category: Plant sciences, botany
Impact factor: 5.360, year: 2018

Seeds derive from ovules upon fertilization and therefore the total number of ovules determines the final seed yield, a fundamental trait in crop plants. Among the factors that co-ordinate the process of ovule formation, the transcription factors CUP-SHAPED COTYLEDON1 (CUC1) and CUC2 and the hormone cytokinin (CK) have a particularly prominent role. Indeed, the absence of both CUC1 and CUC2 causes a severe reduction in ovule number, a phenotype that can be rescued by CK treatment. In this study, we combined CK quantification with an integrative genome-wide target identification approach to select Arabidopsis genes regulated by CUCs that are also involved in CK metabolism. We focused our attention on the functional characterization of UDP-GLUCOSYL TRANSFERASE 85A3 (UGT85A3) and UGT73C1, which are up-regulated in the absence of CUC1 and CUC2 and encode enzymes able to catalyse CK inactivation by O-glucosylation. Our results demonstrate a role for these UGTs as a link between CUCs and CK homeostasis, and highlight the importance of CUCs and CKs in the determination of seed yield.
Permanent Link: http://hdl.handle.net/11104/0288780