Polyphasic data support the splitting of Aspergillus candidus into two species, proposal of Aspergillus dobrogensis sp nov.

Hubka, Vít; Nováková, Alena; Jurjević, Ž.; Sklenář, František; Frisvad, J.C.; Houbraken, J.; Arendrup, M.C.; Jorgensen, K.M.; Sigueira, J.P.Z.; Gené, J.; Kolařík, Miroslav

doi:https://dx.doi.org/10.1099/ijsem.0.002583

Number of the records: 1

Polyphasic data support the splitting of Aspergillus candidus into two species, proposal of Aspergillus dobrogensis sp nov.

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SYSNO ASEP	0494189
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Polyphasic data support the splitting of Aspergillus candidus into two species, proposal of Aspergillus dobrogensis sp nov.
Author(s)	Hubka, Vít (MBU-M)_ORCID Nováková, Alena (MBU-M)_ORCID Jurjević, Ž. (US) Sklenář, František (MBU-M)_ORCID Frisvad, J.C. (DK) Houbraken, J. (NL) Arendrup, M.C. (DK) Jorgensen, K.M. (DK) Sigueira, J.P.Z. (ES) Gené, J. (ES) Kolařík, Miroslav (MBU-M)_{RID, ORCID}
Source Title	International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society - ISSN 1466-5026 Roč. 68, č. 4 (2018), s. 995-1011
Number of pages	17 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	antifungal susceptibility testing ; antioxidant compounds ; bioprospecting
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
R&D Projects	GA17-20286S GA ČR - Czech Science Foundation (CSF)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971
UT WOS	000433170100004
EID SCOPUS	85044924162
DOI	10.1099/ijsem.0.002583
Annotation	Aspergillus candidus is a species frequently isolated from stored grain, food, indoor environments, soil and occasionally also from clinical material. Recent bioprospecting studies highlighted the potential of using A. candidus and its relatives in various industrial sectors as a result of their significant production of enzymes and bioactive compounds. A high genetic variability was observed among A. candidus isolates originating from various European countries and the USA, that were mostly isolated from indoor environments, caves and clinical material. The A. candidus sensu lato isolates were characterized by DNA sequencing of four genetic loci, and agreement between molecular species delimitation results, morphological characters and exometabolite spectra were studied. Classical phylogenetic methods (maximum likelihood, Bayesian inference) and species delimitation methods based on the multispecies coalescent model supported recognition of up to three species in A. candidus sensu lato. After evaluation of phenotypic data, a broader species concept was adopted, and only one new species, Aspergillus dobrogensis, was proposed. This species is represented by 22 strains originating from seven countries (ex-type strain CCF 4651(T) = NRRL 62821(T) = IBT 32697(T) = CBS 143370(T)) and its differentiation from A. candidus is relevant for bioprospecting studies because these species have different exometabolite profiles. Evaluation of the antifungal susceptibility of section Candidi members to six antifungals using the reference EUCAST method showed that all species have low minimum inhibitory concentrations for all tested antifungals. These results suggest applicability of a wide spectrum of antifungal agents for treatment of infections caused by species from section Candidi.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2019

Number of the records: 1