Consequences of gut microbiota manipulation by antibiotics in animal model of early immune dysregulation: phenotype analysis of behaviour and biochemical correlates

Tejkalová, H.; Kačer, P.; Klaschka, Jan; Mrázek, Jakub; Kvasnová, Simona; Fliegerová, Kateřina

Number of the records: 1

Consequences of gut microbiota manipulation by antibiotics in animal model of early immune dysregulation: phenotype analysis of behaviour and biochemical correlates

1.

SYSNO ASEP	0492121
Document Type	A - Abstract
R&D Document Type	The record was not marked in the RIV
R&D Document Type	Není vybrán druh dokumentu
Title	Consequences of gut microbiota manipulation by antibiotics in animal model of early immune dysregulation: phenotype analysis of behaviour and biochemical correlates
Author(s)	Tejkalová, H. (CZ) Kačer, P. (CZ) Klaschka, Jan (UIVT-O)_{RID, SAI, ORCID} Mrázek, Jakub (UZFG-Y)_{RID, ORCID} Kvasnová, Simona (UZFG-Y)_ORCID Fliegerová, Kateřina (UZFG-Y)_{RID, ORCID}
Source Title	FEBS Open Bio . - : Wiley - ISSN 2211-5463 Roč. 8, Suppl. 1 (2018), s. 199-199
Number of pages	1 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	gut microbiome ; microbiome ; rats ; antibiotics ; behaviour ; neurotransmitters
Subject RIV	FH - Neurology
OECD category	Psychiatry
Subject RIV - cooperation	Institute of Animal Physiology and Genetics - Microbiology, Virology
R&D Projects	GBP304/12/G069 GA ČR - Czech Science Foundation (CSF)
	NV17-31852A GA MZd - Ministry of Health (MZ)
Institutional support	UIVT-O - RVO:67985807 ; UZFG-Y - RVO:67985904
UT WOS	000437674102294
Annotation	Host-microbe interactions are now thought as essential for maintaining host health. Additionally, microbiota and activation of host´s immune system play significant role in the development and regulation of CNS. Furthermore, it was presented that certain microbial proteins can mimic several key neuropeptides involved in the regulation of behaviour. The role of the gut microbiome in the bidirectional interaction on the gutbrain axis and the role in a set timeframe for normal synaptogenesis in the development seem crucial. Finally, infectional insult during perinatal period may be responsible for the development of neuropsychiatric disorders such as schizophrenia in later life. According to these facts, our study was aimed at describing behavioural and biochemical alterations in rats exposed to early inflammatory stimulation. This was based on the subchronic administration of lipopolysaccharide to neonatal rat together with effect of microbiota modulation realised by antibiotic cocktail. The10days modulation of gut microbiota was started in adults at PD 60 in drinking water. The microbiota from faeces was analysed before and at the end of experiment together with analysis of neurotransmitters from plasma and brain tissues. Shift in the composition of the bacterial diversity in faeces of studied animals was preliminary assessed by DGGE analysis and then determined by the Ion Torrent nextgeneration sequencing technology. To evaluate changes on low and highmolecular level, the collected biological samples were analysed using advanced metabolomic and proteomic strategies. Significant changes in the fingerprints of bioactive molecules among the tested groups were found. The values were discussed with relation to results obtained in behavioural tests, where impairment of behavioural profiles (locomotion and sensorimotor gating) and weight gain deficit were documented.
Workplace	Institute of Computer Science
Contact	Tereza Šírová, sirova@cs.cas.cz, Tel.: 266 053 800
Year of Publishing	2019

Number of the records: 1