The structural model of Zika virus RNA-dependent RNA polymerase in complex with RNA for rational design of novel nucleotide inhibitors

0492031 - ÚOCHB 2019 RIV GB eng J - Journal Article
Šebera, Jakub - Dubánková, Anna - Sychrovský, Vladimír - Růžek, Daniel - Bouřa, Evžen - Nencka, Radim
The structural model of Zika virus RNA-dependent RNA polymerase in complex with RNA for rational design of novel nucleotide inhibitors.
Scientific Reports. Roč. 8, Jul 24 (2018), č. článku 11132. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR(CZ) GA16-20054S; GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:61388963 ; RVO:60077344
Keywords : Japanese encephalitis virus * hepatitis C virus * crystal structure
OECD category: Virology
Impact factor: 4.011, year: 2018
https://www.nature.com/articles/s41598-018-29459-7

Zika virus is a global health threat due to significantly elevated risk of fetus malformations in infected pregnant women. Currently, neither an effective therapy nor a prophylactic vaccination is available for clinical use, desperately necessitating novel therapeutics and approaches to obtain them. Here, we present a structural model of the Zika virus RNA-dependent RNA polymerase (ZIKV RdRp) in complex with template and nascent RNAs, Mg2+ ions and accessing nucleoside triphosphate. The model allowed for docking studies aimed at effective pre-screening of potential inhibitors of ZIKV RdRp. Applicability of the structural model for docking studies was illustrated with the NITD008 artificial nucleotide that is known to effectively inhibit the function of the ZIKV RdRp. The ZIKV RdRp - RNA structural model is provided for all possible variations of the nascent RNA bases pairs to enhance its general utility in docking and modelling experiments. The developed model makes the rational design of novel nucleosides and nucleotide analogues feasible and thus provides a solid platform for the development of advanced antiviral therapy.
Permanent Link: http://hdl.handle.net/11104/0285614