Does BCA3 Play a Role in the HIV-1 Replication Cycle?

0491247 - ÚOCHB 2019 RIV CH eng J - Journal Article
Rumlová, M. - Křížová, I. - Zelenka, J. - Weber, Jan - Ruml, T.
Does BCA3 Play a Role in the HIV-1 Replication Cycle?
Viruses. Roč. 10, č. 4 (2018), č. článku 212. E-ISSN 1999-4915
Institutional support: RVO:61388963
Keywords : HIV-1 * BCA3 * AKIP-1 * M-PMV * virus incorporation * PKAc
OECD category: Virology
Impact factor: 3.811, year: 2018
http://www.mdpi.com/1999-4915/10/4/212

The cellular role of breast carcinoma-associated protein (BCA3), also known as A-kinase-interacting protein 1 (AKIP-1), is not fully understood. Recently, we reported that full-length, but not C-terminally truncated, BCA3 is incorporated into virions of Mason-Pfizer monkey virus, and that BCA3 enhances HIV-1 protease-induced apoptosis. In the present study, we report that BCA3 is associated with purified and subtilisin-treated HIV particles. Using a combination of immune-based methods and confocal microscopy, we show that the C-terminus of BCA3 is required for packaging into HIV-1 particles. However, we were unable to identify an HIV-1 binding domain for BCA3, and we did not observe any effect of incorporated BCA3 on HIV-1 infectivity. Interestingly, the BCA3 C-terminus was previously identified as a binding site for the catalytic subunit of protein kinase A (PKAc), a cellular protein that is specifically packaged into HIV-1 particles. Based on our analysis of PKAc-BCA3 interactions, we suggest that BCA3 incorporation into HIV-1 particles is mediated by its ability to interact with PKAc.
Permanent Link: http://hdl.handle.net/11104/0285281