0491061 - ÚEB 2019 RIV AT eng J - Journal Article
Angelini, J. - Vosolsobě, S. - Skůpa, Petr - Ho, A. Y. Y. - Bellinvia, E. - Valentová, O. - Marc, J.
Phospholipase D delta assists to cortical microtubule recovery after salt stress.
Protoplasma. Roč. 255, č. 4 (2018), s. 1195-1204. ISSN 0033-183X. E-ISSN 1615-6102
R&D Projects: GA ČR GA14-09685S; GA MŠMT(CZ) LM2015062
Grant - others:OPPK(XE) CZ.2.16/3.1.00/21519
Institutional support: RVO:61389030
Keywords : Arabidopsis roots * by-2 * Microtubule dynamics * Phospholipase Dδ * Salt stress
OECD category: Cell biology
Impact factor: 2.633, year: 2018

The dynamic microtubule cytoskeleton plays fundamental roles in the growth and development of plants including regulation of their responses to environmental stress. Plants exposed to hyper-osmotic stress commonly acclimate, acquiring tolerance to variable stress levels. The underlying cellular mechanisms are largely unknown. Here, we show, for the first time, by in vivo imaging approach that linear patterns of phospholipase Dδ match the localization of microtubules in various biological systems, validating previously predicted connection between phospholipase Dδ and microtubules. Both the microtubule and linear phospholipase Dδ structures were disintegrated in a few minutes after treatment with oryzalin or salt. Moreover, by using immunofluorescence confocal microscopy of the cells in the root elongation zone of Arabidopsis, we have shown that the cortical microtubules rapidly depolymerized within 30 min of treatment with 150 or 200 mM NaCl. Within 5 h of treatment, the density of microtubule arrays was partially restored. A T-DNA insertional mutant lacking phospholipase Dδ showed poor recovery of microtubule arrays following salt exposition. The restoration of microtubules was significantly retarded as well as the rate of root growth, but roots of overexpressor GFP-PLDδ prepared in our lab, have grown slightly better compared to wild-type plants. Our results indicate that phospholipase Dδ is involved in salt stress tolerance, possibly by direct anchoring and stabilization of de novo emerging microtubules to the plasma membrane, providing novel insight into common molecular mechanism during various stress events.
Permanent Link: http://hdl.handle.net/11104/0285146