Number of the records: 1
Capillary Network Morphometry of Pig Soleus Muscle Significantly Changes in 24 Hours After Death
- 1.
SYSNO ASEP 0490241 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Capillary Network Morphometry of Pig Soleus Muscle Significantly Changes in 24 Hours After Death Author(s) Eržen, I. (SI)
Janáček, Jiří (FGU-C) RID, ORCID
Kreft, M. (SI)
Kubínová, Lucie (FGU-C) RID, ORCID
Cvetko, E. (SI)Source Title Journal of Histochemistry and Cytochemistry. - : Sage - ISSN 0022-1554
Roč. 66, č. 1 (2018), s. 23-31Number of pages 9 s. Language eng - English Country US - United States Keywords capillaries ; confocal microscopy ; image analysis ; pig ; postmortem changes ; skeletal muscle Subject RIV FP - Other Medical Disciplines OECD category Anatomy and morphology (plant science to be 1.6) R&D Projects LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FGU-C - RVO:67985823 UT WOS 000418835500002 EID SCOPUS 85039732994 DOI 10.1369/0022155417737061 Annotation Capillary network characteristics are invaluable for diagnostics of muscle diseases. Biopsy material is limited in size and mostly not accessible for intensive research. Therefore, especially in human tissue, studies are performed on autopsy material. To approach the problem whether it is reliable to deduce hypotheses from autopsy material to explain physiological and pathological processes, we studied capillarity in pig soleus muscle 1 and 24 hr after death. Capillaries and muscle fibers were immunofluorescently marked, and images were acquired with a confocal microscope. Characteristics of the capillary network were estimated by image analysis methods using several plugins of the Ellipse program. Twenty-four hours after death, the measured characteristics of the capillary network differ by up to 50% when compared with samples excised 1 hr after death. Muscle fiber diameter, the measured capillary length, and tortuosity were reduced, and capillary network became more anisotropic. The main postmortem change that affects capillaries is evidently geometric deformation of muscle tissue. In conclusion, when comparing results from biopsy samples with those from autopsy samples, the effect of postmortem changes on the measured parameters must be carefully considered. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2019
Number of the records: 1