Manipulating the mitochondria activity in human hepatic cell line Huh7 by low-power laser irradiation

0489933 - FZÚ 2019 RIV US eng J - Journal Article
Lynnyková, Anna - Lunova, Mariia - Jirsa, M. - Egorova, D. - Kulikov, A. - Kubinová, Šárka - Lunov, Oleg - Dejneka, Alexandr
Manipulating the mitochondria activity in human hepatic cell line Huh7 by low-power laser irradiation.
Biomedical Optics Express. Roč. 9, č. 3 (2018), s. 1283-1300. ISSN 2156-7085
Institutional support: RVO:68378271
Keywords : manipulating * mitochondria activity * human hepatic cell * line Huh7 * low-power
OECD category: Biophysics
Impact factor: 3.910, year: 2018

Low-power laser irradiation of red light has been recognized as a promising tool across a vast variety of biomedical applications. However, deep understanding of the molecular mechanisms behind laser-induced cellular effects remains a significant challenge. Here, we investigated mechanisms involved in the death process in human hepatic cell line Huh7 at a laser irradiation. We decoupled distinct cell death pathways targeted by laser irradiations of different powers. Our data demonstrate that high dose laser irradiation exhibited the highest levels of total reactive oxygen species production, leading to cyclophilin D-related necrosis via the mitochondrial permeability transition. On the contrary, low dose laser irradiation resulted in the nuclear accumulation of superoxide and apoptosis execution.
Permanent Link: http://hdl.handle.net/11104/0284231