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The Biological Role of Hyaluronan-Rich Oocyte-Cumulus Extracellular Matrix in Female Reproduction
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SYSNO ASEP 0489476 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Biological Role of Hyaluronan-Rich Oocyte-Cumulus Extracellular Matrix in Female Reproduction Author(s) Nagyová, Eva (UZFG-Y) RID, ORCID Article number 283 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 19, č. 1 (2018)Number of pages 14 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords extracellular matrix ; hyaluronan ; inter-alpha-trypsin inhibitor Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UZFG-Y - RVO:67985904 UT WOS 000424407200277 EID SCOPUS 85040944455 DOI 10.3390/ijms19010283 Annotation Fertilization of the mammalian oocyte requires interactions between spermatozoa and expanded cumulus extracellular matrix (ECM) that surrounds the oocyte. This review focuses on key molecules that play an important role in the formation of the cumulus ECM, generated by the oocyte-cumulus complex. In particular, the specific inhibitors (AG1478, lapatinib, indomethacin and MG132) and progesterone receptor antagonist (RU486) exerting their effects through the remodeling of the ECM of the cumulus cells surrounding the oocyte have been described. After gonadotropin stimulus, cumulus cells expand and form hyaluronan (HA)-rich cumulus ECM. In pigs, the proper structure of the cumulus ECM depends on the interaction between HA and serum-derived proteins of the inter-alpha-trypsin inhibitor (II) protein family. We have demonstrated the synthesis of HA by cumulus cells, and the presence of the II, tumor necrosis factor-alpha-induced protein 6 and pentraxin 3 in expanding oocyte-cumulus complexes (OCC). We have evaluated the covalent linkage of heavy chains of II proteins to HA, as the principal component of the expanded HA-rich cumulus ECM, in porcine OCC cultured in medium with specific inhibitors: AG1478 and lapatinib (both inhibitors of epidermal growth factor receptor tyrosine kinase activity), MG132 (a specific proteasomal inhibitor), indomethacin (cyclooxygenase inhibitor), and progesterone receptor antagonist (RU486). We have found that both RU486 and indomethacin does not disrupt the formation of the covalent linkage between the heavy chains of II to HA in the expanded OCC. In contrast, the inhibitors AG1478 and lapatinib prevent gonadotropin-induced cumulus expansion. Finally, the formation of oocyte-cumulus ECM relying on the covalent transfer of heavy chains of II molecules to HA has been inhibited in the presence of MG132. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2019
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