Number of the records: 1  

The Biological Role of Hyaluronan-Rich Oocyte-Cumulus Extracellular Matrix in Female Reproduction

  1. 1.
    SYSNO ASEP0489476
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleThe Biological Role of Hyaluronan-Rich Oocyte-Cumulus Extracellular Matrix in Female Reproduction
    Author(s) Nagyová, Eva (UZFG-Y) RID, ORCID
    Article number283
    Source TitleInternational Journal of Molecular Sciences. - : MDPI
    Roč. 19, č. 1 (2018)
    Number of pages14 s.
    Publication formOnline - E
    Languageeng - English
    CountryCH - Switzerland
    Keywordsextracellular matrix ; hyaluronan ; inter-alpha-trypsin inhibitor
    Subject RIVEB - Genetics ; Molecular Biology
    OECD categoryBiochemistry and molecular biology
    R&D ProjectsEF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportUZFG-Y - RVO:67985904
    UT WOS000424407200277
    EID SCOPUS85040944455
    DOI10.3390/ijms19010283
    AnnotationFertilization of the mammalian oocyte requires interactions between spermatozoa and expanded cumulus extracellular matrix (ECM) that surrounds the oocyte. This review focuses on key molecules that play an important role in the formation of the cumulus ECM, generated by the oocyte-cumulus complex. In particular, the specific inhibitors (AG1478, lapatinib, indomethacin and MG132) and progesterone receptor antagonist (RU486) exerting their effects through the remodeling of the ECM of the cumulus cells surrounding the oocyte have been described. After gonadotropin stimulus, cumulus cells expand and form hyaluronan (HA)-rich cumulus ECM. In pigs, the proper structure of the cumulus ECM depends on the interaction between HA and serum-derived proteins of the inter-alpha-trypsin inhibitor (II) protein family. We have demonstrated the synthesis of HA by cumulus cells, and the presence of the II, tumor necrosis factor-alpha-induced protein 6 and pentraxin 3 in expanding oocyte-cumulus complexes (OCC). We have evaluated the covalent linkage of heavy chains of II proteins to HA, as the principal component of the expanded HA-rich cumulus ECM, in porcine OCC cultured in medium with specific inhibitors: AG1478 and lapatinib (both inhibitors of epidermal growth factor receptor tyrosine kinase activity), MG132 (a specific proteasomal inhibitor), indomethacin (cyclooxygenase inhibitor), and progesterone receptor antagonist (RU486). We have found that both RU486 and indomethacin does not disrupt the formation of the covalent linkage between the heavy chains of II to HA in the expanded OCC. In contrast, the inhibitors AG1478 and lapatinib prevent gonadotropin-induced cumulus expansion. Finally, the formation of oocyte-cumulus ECM relying on the covalent transfer of heavy chains of II molecules to HA has been inhibited in the presence of MG132.
    WorkplaceInstitute of Animal Physiology and Genetics
    ContactJana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
    Year of Publishing2019
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.