i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions

Dvořáková, Zuzana; Renčiuk, Daniel; Kejnovská, Iva; Školáková, Petra; Bednářová, Klára; Sagi, J.; Vorlíčková, Michaela

doi:https://dx.doi.org/10.1093/nar/gky035

Number of the records: 1

i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions

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SYSNO ASEP	0488376
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions
Author(s)	Dvořáková, Zuzana (BFU-R)_ORCID Renčiuk, Daniel (BFU-R)_{RID, ORCID} Kejnovská, Iva (BFU-R)_{RID, ORCID} Školáková, Petra (BFU-R)_ORCID Bednářová, Klára (BFU-R)_ORCID Sagi, J. (US) Vorlíčková, Michaela (BFU-R)_{RID, ORCID}
Number of authors	7
Source Title	Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048 Roč. 46, č. 4 (2018), s. 1624-1634
Number of pages	11 s.
Publication form	Print - P
Language	eng - English
Country	GB - United Kingdom
Keywords	pair opening kinetics ; g-quadruplex dna
Subject RIV	CE - Biochemistry
OECD category	Biochemistry and molecular biology
R&D Projects	GA15-06785S GA ČR - Czech Science Foundation (CSF)
	GA17-12075S GA ČR - Czech Science Foundation (CSF)
	GJ17-19170Y GA ČR - Czech Science Foundation (CSF)
	EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BFU-R - RVO:68081707
UT WOS	000426293300012
DOI	https://doi.org/10.1093/nar/gky035
Annotation	i-Motif (iM) is a four stranded DNA structure formed by cytosine-rich sequences, which are often present in functionally important parts of the genome such as promoters of genes and telomeres. Using electronic circular dichroism and UV absorption spectroscopies and electrophoretic methods, we examined the effect of four naturally occurring DNA base lesions on the folding and stability of the iM formed by the human telomere DNA sequence (C3TAA) 3C3T. The results demonstrate that the TAA loop lesions, the apurinic site and 8-oxoadenine substituting for adenine, and the 5-hydroxymethyluracil substituting for thymine only marginally disturb the formation of iM. The presence of uracil, which is formed by enzymatic or spontaneous deamination of cytosine, shifts iM formation towards substantially more acidic pH values and simultaneously distinctly reduces iM stability. This effect depends on the position of the damage sites in the sequence. The results have enabled us to formulate additional rules for iM formation.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2018

Number of the records: 1