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Changes in plasma membrane aquaporin gene expression under osmotic stress and blue light in tomato
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SYSNO ASEP 0487922 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Changes in plasma membrane aquaporin gene expression under osmotic stress and blue light in tomato Author(s) Balarynová, Jana (UEB-Q)
Danihlík, J. (CZ)
Fellner, Martin (UEB-Q) RID, ORCIDNumber of authors 3 Article number 27 Source Title Acta Physiologiae Plantarum. - : Springer - ISSN 0137-5881
Roč. 40, č. 2 (2018)Number of pages 8 s. Language eng - English Country PL - Poland Keywords male-sterile mutant ; arabidopsis-thaliana ; seed-germination ; abscisic-acid ; solanum-lycopersicon ; nitric-oxide ; 7b-1 ; protein ; hypocotyl ; responses ; Tomato ; Seed ; Aquaporins ; Blue light ; 7B-1 mutant ; Mannitol ; PIPs Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000424497300006 DOI 10.1007/s11738-017-2602-7 Annotation Divergent abiotic stresses induce osmotic stress on plant cells resulting in an imbalance in water homeostasis which is preserved by aquaporins. Since the plasma membrane aquaporins (PIPs) were shown to be involved in seed development and responses to abiotic stresses, we focused on determining the contribution of mannitol-induced osmotic stress, blue light (BL), and 7B-1 mutation to their gene expression in tomato (Solanum lycopersicum L.) seeds. To assess that, we used a quantitative RT-PCR to determine the expression profiles of genes encoding PIPs. Subsequently, a multiple linear regression analysis was used to evaluate the impact of studied stressors (mannitol and BL) and 7B-1 mutation on PIP gene expressions. We found that mannitol-induced osmotic stress and 7B-1 mutation (conferring the lower responsiveness to osmotic stress-and BL-induced inhibition of seed germination) decreased expression of PIP1, 3, PIP2, 3 and PIP1, 2, PIP2, 1 genes, respectively. This might be a way to retain water for radicle elongation and seed germination under the stress conditions. Interestingly, the expression of PIP1, 3 gene was downregulated not only by osmotic stress, but also by BL. Altogether, our data indicate the existence of a link between osmotic stress and BL signalling and the involvement of the 7B-1 mutation in this crosstalk. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2019
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