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Analysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery
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SYSNO ASEP 0487351 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery Author(s) Živicová, V. (CZ)
Lacina, L. (CZ)
Mateu, R. (CZ)
Smetana, K. (CZ)
Kavkova, R. (CZ)
Krejci, E.D. (CZ)
Grim, M. (CZ)
Kvasilová, A. (CZ)
Borský, J. (CZ)
Strnad, Hynek (UMG-J) RID
Hradilová, Miluše (UMG-J)
Šáchová, Jana (UMG-J)
Kolář, Michal (UMG-J) RID, ORCID
Dvořánková, B. (CZ)Number of authors 14 Source Title International Journal of Molecular Medicine. - : Spandidos Publications - ISSN 1107-3756
Roč. 40, č. 5 (2017), s. 1323-1334Number of pages 12 s. Language eng - English Country GR - Greece Keywords dermal fibroblasts ; myofibroblast ; neonatal healing ; trandforming growth factor-beta ; cleft Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology R&D Projects GA13-20293S GA ČR - Czech Science Foundation (CSF) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 UT WOS 000413398800003 DOI 10.3892/ijmm.2017.3128 Annotation The nonsyndromic cleft is one of the most frequent congenital defects in humans. Clinical data demonstrated improved and almost scarless neonatal healing of reparative surgery. Based on our previous results on crosstalk between neonatal fibroblasts and adult keratinocytes, the present study focused on characterization of fibroblasts prepared from cleft lip tissue samples of neonates and older children, and compared them with samples isolated from normal adult skin (face and breast) and scars. Although subtle variances in expression profiles of children and neonates were observed, the two groups differed significantly from adult cells. Compared with adult cells, differences were observed in nestin and smooth muscle actin (SMA) expression at the protein and transcript level. Furthermore, fibroblast to myofibroblast differentiation drives effective wound healing and is largely regulated by the cytokine, transforming growth factor-beta 1 (TGF-beta 1). Dysregulation of the TGF-beta signalling pathway, including low expression of the TGF-beta receptor II, may contribute to reducing scarring in neonates. Fibroblasts of facial origin also exhibited age independent differences from the cells prepared from the breast, reflecting the origin of the facial cells from neural crest-based ectomesenchyme. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2018
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