Number of the records: 1
Wheat Rust Diseases
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SYSNO ASEP 0485924 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Rapid gene isolation using MutChromSeq Author(s) Steuernagel, B. (GB)
Vrána, Jan (UEB-Q) RID, ORCID
Karafiátová, Miroslava (UEB-Q) RID, ORCID
Wulff, B. B. H. (GB)
Doležel, Jaroslav (UEB-Q) RID, ORCIDNumber of authors 5 Source Title Wheat Rust Diseases. - New York : Humana Press, 2017 / Periyannan S. - ISSN 1064-3745 - ISBN 978-1-4939-7248-7 Pages roč. 1659 (2017), s. 231-243 Number of pages 13 s. Number of copy 2600 Number of pages 294 Publication form Print - P Language eng - English Country US - United States Keywords Chromosome isolation ; fishis ; Flow cytometry and chromosome sorting ; Gene cloning ; Mutational genomics Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000431343700021 EID SCOPUS 85028985949 DOI 10.1007/978-1-4939-7249-4_20 Annotation MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018 Electronic address http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=28856655
Number of the records: 1