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Size-exclusion chromatography and its optimization for material science
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SYSNO ASEP 0481739 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Size-exclusion chromatography and its optimization for material science Author(s) Netopilík, Miloš (UMCH-V) RID
Trhlíková, Olga (UMCH-V) RID, ORCIDArticle number 358 Source Title Journal of Material Sciences & Engineering - ISSN 2169-0022
Roč. 6, č. 4 (2017), s. 1-5Number of pages 5 s. Language eng - English Country US - United States Keywords separation mechanism ; size-exclusion chromatography ; band broadening function Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects GC17-04258J GA ČR - Czech Science Foundation (CSF) Institutional support UMCH-V - RVO:61389013 DOI 10.4172/2169-0022.1000358 Annotation A theoretical analysis of improving the separation power of size exclusion chromatography by decreasing flowrate is conveyed. The variance of the elution curves is larger than expected form estimated dispersity in molecular weight, Mw/Mn. The shape of the elution curves depends strongly on experimental conditions. When the experimental conditions are approaching those to the ideal separation, judged by statistical properties of the curves, both skew and excess kurtosis of the elution curves increase near the exclusion limit in accord with theoretical prediction. In analyses of polystyrene standards by size exclusion chromatography for polymers of molecular weight about thirty thousand and higher, the longitudinal diffusion is not important even at low flow-rates. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2018
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