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Effect of hyperbaric air on endotoxin from Bacteroides fragilis strains
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SYSNO ASEP 0481405 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effect of hyperbaric air on endotoxin from Bacteroides fragilis strains Author(s) Chmelař, D. (CZ)
Kašíková, A. (CZ)
Martineková, P. (CZ)
Hájek, M. (CZ)
Rozložník, M. (CZ)
Brabec, Marek (UIVT-O) RID, SAI, ORCID
Janečková, J. (CZ)
Vobejdová, J. (CZ)
Čižnár, I. (SK)Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 63, č. 3 (2018), s. 283-290Number of pages 8 s. Language eng - English Country CZ - Czech Republic Keywords endotoxin ; hyperbaric condition Subject RIV EE - Microbiology, Virology OECD category Microbiology Institutional support UIVT-O - RVO:67985807 UT WOS 000430317100003 EID SCOPUS 85033576347 DOI https://doi.org/10.1007/s12223-017-0564-1 Annotation The aim of the project was to determine any effect of hyperbaric air on Bacteroides fragilis strains cultivated under hyperbaric conditions. Previously, it was hypothesized that there was a correlation between the presence of Bacteroides bacteria in patients preferring a meaty diet and cancer of the small intestine, and particularly of the large intestine and rectum. With respect to the fact that Bacteroides fragilis (BAFR) group are important producers of endotoxins, measurement and statistical evaluation of endotoxin production by individual strains of isolated Bacteroides species were used to compare bacteria isolated from various clinical samples from patients with colon and rectum cancer in comparison with strains isolated from other non-cancer diagnoses. Endotoxin production was proven by quantitative detection using the limulus amebocyte lysate (LAL) test in EU/mL. Production of endotoxins in these bacteria cultured under hyperbaric air conditions was higher than those strains cultured under normobaric anaerobic conditions. But these differences in endotoxin production were not statistically significant (t test with log-transformed data, p value = 0.0910). Based on a two-tier t test for lognormal data, it is possible to cautiously conclude that a statistically significant difference was found between endotoxin production by Bacteroides fragilis strains isolated from non-carcinoma diagnoses (strains (1–6) and strains isolated from colorectal carcinoma diagnoses (strains 7–8, Wilcoxon non-parametric test p = 0.0132, t test = 0.1110, t test with log-transformed data, p value = 0.0294). Workplace Institute of Computer Science Contact Tereza Šírová, sirova@cs.cas.cz, Tel.: 266 053 800 Year of Publishing 2019
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