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Auxins and Cytokinins in Plant Biology: Methods and Protocols
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SYSNO ASEP 0480370 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Analytical determination of auxins and cytokinins Author(s) Dobrev, Petre (UEB-Q) RID, ORCID
Hoyerová, Klára (UEB-Q) RID, ORCID
Petrášek, Jan (UEB-Q) RID, ORCIDNumber of authors 3 Source Title Auxins and Cytokinins in Plant Biology: Methods and Protocols. - Dordrecht : Springer, 2017 / Dandekar T. ; Naseem M. - ISSN 1064-3745 - ISBN 978-1-4939-6831-2 Pages s. 31-39 Number of pages 9 s. Number of copy 5000 Number of pages 204 Publication form Print - P Language eng - English Country NL - Netherlands Keywords Auxin ; Cytokinins ; Liquid chromatography ; Mass spectrometry ; Phytohormones Subject RIV ED - Physiology OECD category Analytical chemistry R&D Projects LD14120 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LD15137 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000411979900003 EID SCOPUS 85014774144 DOI 10.1007/978-1-4939-6831-2_2 Annotation Parallel determination of auxin and cytokinin levels within plant organs and tissues represents an invaluable tool for studies of their physiological effects and mutual interactions. Thanks to their different chemical structures, auxins, cytokinins and their metabolites are often determined separately, using specialized procedures of sample purification, extraction, and quantification. However, recent progress in the sensitivity of analytical methods of liquid chromatography coupled to mass spectrometry (LC-MS) allows parallel analysis of multiple compounds. Here we describe a method that is based on single step purification protocol followed by LC-MS separation and detection for parallel analysis of auxins, cytokinins and their metabolites in various plant tissues and cell cultures. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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