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Transcriptomic Response of Arabidopsis thaliana Exposed to CuO Nanoparticles, Bulk Material, and Ionic Copper
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SYSNO ASEP 0479928 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transcriptomic Response of Arabidopsis thaliana Exposed to CuO Nanoparticles, Bulk Material, and Ionic Copper Author(s) Landa, Přemysl (UEB-Q) RID, ORCID
Dytrych, Pavel (UCHP-M) RID, ORCID, SAI
Přerostová, Sylva (UEB-Q) RID, ORCID, SAI
Petrová, Šárka (UEB-Q) RID, ORCID
Vaňková, Radomíra (UEB-Q) RID, ORCID
Vaněk, Tomáš (UEB-Q) RID, ORCIDNumber of authors 6 Source Title Environmental Science and Technology - ISSN 0013-936X
Roč. 51, č. 18 (2017), s. 10814-10824Number of pages 11 s. Language eng - English Country US - United States Keywords METAL-OXIDE NANOPARTICLES ; GENE-EXPRESSION ; JASMONIC ACID Subject RIV DN - Health Impact of the Environment Quality OECD category Plant sciences, botany Subject RIV - cooperation Institute of Chemical Process Fundamentals - Industrial Chemistry, Chemical Engineering R&D Projects LD14125 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 8G15003 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 ; UCHP-M - RVO:67985858 UT WOS 000411549800060 EID SCOPUS 85029690803 DOI 10.1021/acs.est.7b02265 Annotation Engineered nanoparticles (ENPs) exhibit unique properties advantageous in a number of applications, but they also represent potential health and environmental risks. In this study, we investigated the phytotoxic mechanism of CuO ENPs using transcriptomic analysis and compared this response with the response to CuO bulk particles and ionic Cu 2+ . Ionic Cu 2+ at the concentration of 0.16 mg L1 changed transcription of 2692 genes (p value of < 0.001, fold change of ≥2) after 7 days of exposure, whereas CuO ENPs and bulk particles (both in the concentration of 10 mg L1 ) altered the expression of 922 and 482 genes in Arabidopsis thaliana roots, respectively. The similarity between transcription profiles of plants exposed to ENPs and ionic Cu 2+ indicated that the main factor in phytotoxicity was the release of Cu 2+ ions from CuO ENPs after 7 days of exposure. The effect of Cu 2+ ions was evident in all treatments, as indicated by the down-regulation of genes involved in metal homeostasis and transport and the up-regulation of oxidative stress response genes. ENPs were more soluble than bulk particles, resulting in the up-regulation of metallochaperone-like genes or the down-regulation of aquaporins and metal transmembrane transporters that was also characteristic for ionic Cu 2+ exposure. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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