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Physiological roles of sigma factor SigD in Corynebacterium glutamicum
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SYSNO ASEP 0477066 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Physiological roles of sigma factor SigD in Corynebacterium glutamicum Author(s) Taniguchi, H. (DE)
Busche, T. (DE)
Patschkowski, T. (DE)
Niehaus, K. (DE)
Pátek, Miroslav (MBU-M) RID, ORCID
Kalinowski, J. (DE)
Wendisch, V.F. (DE)Source Title BMC Microbiology. - : BioMed Central - ISSN 1471-2180
Roč. 17, č. 158 (2017), s. 158Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords Corynebacterium glutamicum ; Sigma factor ; SigD Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA17-06991S GA ČR - Czech Science Foundation (CSF) Institutional support MBU-M - RVO:61388971 UT WOS 000405422000002 EID SCOPUS 85023166489 DOI https://doi.org/10.1186/s12866-017-1067-6 Annotation Background: Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail, however, the role of SigD in transcriptional regulation in C. glutamicum has been mostly unknown.
Results: In this work, pleiotropic effects of sigD overexpression at the level of phenotype, transcripts, proteins and metabolites were investigated. Overexpression of sigD decreased the growth rate of C. glutamicum cultures, and induced several physiological effects such as reduced culture foaming, turbid supernatant and cell aggregation. Upon overexpression of sigD, the level of Cmt1 (corynomycolyl transferase) in the supernatant was notably enhanced, and carbohydrate-containing compounds were excreted to the supernatant. The real-time PCR analysis revealed that sigD overexpression increased the expression of genes related to corynomycolic acid synthesis (fadD2, pks), genes encoding corynomycolyl transferases (cop1, cmt1, cmt2, cmt3), L, D-transpeptidase (lppS), a subunit of the major cell wall channel (porH), and the envelope lipid regulation factor (elrF). Furthermore, overexpression of sigD resulted in trehalose dicorynomycolate accumulation in the cell envelope.
Conclusions: This study demonstrated that SigD regulates the synthesis of corynomycolate and related compounds, and expanded the knowledge of regulatory functions of sigma factors in C. glutamicum.Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2018
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