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Phytochemical Characterization, Antibacterial, Acetylcholinesterase Inhibitory and Cytotoxic Properties of Cryptostephanus vansonii, an Endemic Amaryllid
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SYSNO ASEP 0476489 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Phytochemical Characterization, Antibacterial, Acetylcholinesterase Inhibitory and Cytotoxic Properties of Cryptostephanus vansonii, an Endemic Amaryllid Author(s) Moyo, M. (ZA)
Aremu, A.O. (ZA)
Chukwujekwu, J. C. (ZA)
Grúz, Jiří (UEB-Q) RID, ORCID
Skořepa, Jiří (UEB-Q)
Doležal, Karel (UEB-Q) RID, ORCID
Katsvanga, C. A. T. (ZW)
Van Staden, J. (ZA)Number of authors 8 Source Title Phytotherapy Research - ISSN 0951-418X
Roč. 31, č. 5 (2017), s. 713-720Number of pages 8 s. Language eng - English Country US - United States Keywords in-vitro ; alzheimers-disease ; alkaloids ; extracts ; antioxidant ; coumarins ; apoptosis ; phylogeny ; medicine ; bacteria ; acetylcholinesterase inhibition ; Amaryllidaceae ; antibacterial ; cytotoxicity ; flavonoids ; phenolic acids Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LK21306 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000400149200002 DOI 10.1002/ptr.5788 Annotation Cryptostephanus vansonii I. Verd., an endemic Amaryllidaceae species from Zimbabwe, was evaluated for its acetylcholinesterase (AChE) inhibitory and cytotoxicity properties using Ellman's colorimetric method and the tetrazolium-based colorimetric assay against Vero monkey kidney cells, respectively. The plant extracts were also evaluated for their antibacterial activity against five bacteria. Furthermore, phytochemical profiles of the extracts were determined using ultra-high performance liquid chromatography coupled with tandem mass spectrometry analysis. A plant part-dependent AChE inhibitory activity was observed, in the order, root>rhizome>basal leaf>leaf. Overall, C. vansonii extracts exhibited better antibacterial activity against Gram-negative compared with Gram-positive bacteria. Cytotoxic effects were not detected in Vero monkey kidney cell lines suggesting the possible absence of toxophores in C. vansonii extracts. Similar to the trend in biological activity, a distinct plant part-dependent variation in hydroxybenzoates, hydroxycinnamates and flavonoids was observed in the plant extracts. In addition, 5-hydroxymetylfurfural and eucomic acid were detected in the different plant parts of C. vansonii. The results of the present study provide valuable AChE inhibition activity, toxicological and phytochemical profiles of C. vansonii. Further studies on isolation of bioactive compounds and their subsequent evaluation in other pharmacological and toxicological model systems are required. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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