Concentration of nucleic acids by couplad column capillary isotachophoresis application to circulating cell-free dna extraction from liquid biopsy samples

0475779 - ÚIACH 2018 CZ eng A - Abstract
Datinská, Vladimíra - Voráčová, Ivona - Schlecht, U. - Berka, J. - Foret, František
Concentration of nucleic acids by couplad column capillary isotachophoresis application to circulating cell-free dna extraction from liquid biopsy samples.
HPLC 2017. Final Program. Praha: Czech-In s. r. o., 2017. s. 227-228.
[International symposium on high performance liquid phase separations and related techniques /45./. 18.06.2017-22.06.2017, Praha]
Institutional support: RVO:68081715
Keywords : nucleic acids * DNA extraction * cell-free DNA
Subject RIV: CB - Analytical Chemistry, Separation

Isotachophoresis is an easy-to-automate electrophoretic method of high robustness and sensitivity with remarkable potential for sample pre-concentration, purification and buffer exchange. In this work, the commercial ITP instrument was tested for pre-concentration of DNA.
Permanent Link: http://hdl.handle.net/11104/0272408