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Novel resistance to Cydia pomonella granulovirus (CpGV) in codling moth shows autosomal and dominant inheritance and confers cross-resistance to different CpGV genome groups
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SYSNO ASEP 0475637 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Novel resistance to Cydia pomonella granulovirus (CpGV) in codling moth shows autosomal and dominant inheritance and confers cross-resistance to different CpGV genome groups Author(s) Sauer, A. J. (DE)
Fritsch, E. (DE)
Undorf-Spahn, K. (DE)
Nguyen, Petr (BC-A) RID, ORCID
Marec, František (BC-A) RID, ORCID
Heckel, D. G. (DE)
Jehle, J. A. (DE)Number of authors 7 Article number e0179157 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 12, č. 6 (2017)Number of pages 17 s. Language eng - English Country US - United States Keywords Cydia pomonella granulovirus ; inheritance ; resistance Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects GA14-22765S GA ČR - Czech Science Foundation (CSF) GP14-35819P GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 UT WOS 000404135800019 EID SCOPUS 85021206512 DOI 10.1371/journal.pone.0179157 Annotation Commercial Cydia pomonella granulovirus (CpGV) products have been successfully applied to control codling moth (CM) in organic and integrated fruit production for more than 30 years. Since 2005, resistance against the widely used isolate CpGV-M has been reported from different countries in Europe. The inheritance of this so-called type I resistance is dominant and linked to the Z chromosome. Recently, a second form (type II) of CpGV resistance in CM was reported from a field population (NRW-WE) in Germany. Type II resistance confers reduced susceptibility not only to CpGV-M but to most known CpGV isolates and it does not follow the previously described Z-linked inheritance of type I resistance. To further analyze type II resistance, two CM strains, termed CpR5M and CpR5S, were generated from parental NRW-WE by repeated mass crosses and selection using the two isolates CpGV-M and CpGV-S, respectively. Both CpR5M and CpR5S were considered to be genetically homogeneous for the presence of the resistance allele(s). By crossing and backcrossing experiments with a susceptible CM strain, followed by resistance testing of the offspring, an autosomal dominant inheritance of resistance was elucidated. In addition, cross-resistance to CpGV-M and CpGV-S was detected in both strains, CpR5M and CpR5S. To test the hypothesis that the autosomal inheritance of type II resistance was caused by a large interchromosomal rearrangement involving the Z chromosome, making type I resistance appear to be autosomal in these strains, fluorescence in situ hybridization with bacterial artificial chromosome probes (BAC-FISH) was used to physically map the Z chromosomes of different CM strains. Conserved synteny of the Z-linked genes in CpR5M and other CM strains rejects this hypothesis and argues for a novel genetic and functional mode of resistance in CM populations with type II resistance. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2018 Electronic address http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0179157
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