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G-quadruplex formation in the Oct4 promoter positively regulates Oct4 expression
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SYSNO ASEP 0473579 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title G-quadruplex formation in the Oct4 promoter positively regulates Oct4 expression Author(s) Renčiuk, Daniel (BFU-R) RID, ORCID
Ryneš, J. (CZ)
Kejnovská, Iva (BFU-R) RID, ORCID
Foldynova-Trantirkova, S. (CZ)
Andaeng, M. (SE)
Trantírek, L. (CZ)
Vorlíčková, Michaela (BFU-R) RID, ORCIDNumber of authors 7 Source Title Biochimica et Biophysica Acta-Gene Regulatory Mechanisms. - : Elsevier - ISSN 1874-9399
Roč. 1860, č. 2 (2017), s. 175-183Number of pages 9 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords linked polymorphic region ; guanine quadruplexes ; transcription factor Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects GP14-33947P GA ČR - Czech Science Foundation (CSF) GAP205/12/0466 GA ČR - Czech Science Foundation (CSF) GA15-06785S GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000394072900001 DOI 10.1016/j.bbagrm.2016.11.002 Annotation The Oct4 gene codes for a transcription factor that plays a critical role in the maintenance of pluripotency in embryonic and cancer stem cells. Its expression thus has to be tightly regulated. We performed biophysical characterization of the promoter region using a combination of UV absorption, CD, and NMR spectroscopies, native PAGE and chemical probing, which was followed by functional studies involving luciferase reporter assays performed in osteosarcoma and human embryonic stem cell lines. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2018
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