0472681 - ÚFCH JH 2017 RIV US eng J - Journal Article
Ibanez, D. - Santidrian, Ana - Heras, A. - Kalbáč, Martin - Colina, A.
Study of Adenine and Guanine Oxidation Mechanism by Surface-Enhanced Raman Spectroelectrochemistry.
Journal of Physical Chemistry C. Roč. 119, č. 15 (2015), s. 8191-8198. ISSN 1932-7447. E-ISSN 1932-7455
R&D Projects: GA MŠMT LL1301
Institutional support: RVO:61388955
Keywords : nucleic-acid bases * electrochemical oxidation * silver electrode
Subject RIV: CG - Electrochemistry
Impact factor: 4.509, year: 2015 ; AIS: 1.17, rok: 2015
DOI: https://doi.org/10.1021/acs.jpcc.5b00938

Metal nanoparticles are systems largely employed in surface-enhanced Raman spectroscopy (SERS). In particular, gold nanoparticles are one of the best substrates used in this field. In this work, the optimal conditions for gold nanoparticles electrodeposition on single-walled carbon nanotubes electrodes have been established to obtain the best SERS response. Using this substrate and analyzing the changes of in situ Raman spectra obtained at different potentials, we have been able to explain simultaneously the oxidation mechanism of purine bases, differentiating the oxidation intermediates and their orientation during the different oxidation steps. Adenine orientation hardly changes during the whole oxidation; the molecule maintains a parallel configuration and only shows a slightly tilted orientation after the first oxidation step. On the other hand, guanine orientation changes completely during its oxidation. Initially, guanine is perpendicular respect to gold nanoparticles, changing its orientation after the first oxidation process when the molecule shows a slightly tilted orientation, and it finishes parallel respect to the electrode surface after the second oxidation step.
Permanent Link: http://hdl.handle.net/11104/0269915