0471980 - BFÚ 2017 RIV GB eng J - Journal Article
Dorčák, Vlastimil - Kabeláč, M. - Kroutil, O. - Bednářová, Klára - Vacek, J.
Electrocatalytic monitoring of peptidic proton-wires.
Analyst. Roč. 141, č. 15 (2016), s. 4554-4557. ISSN 0003-2654. E-ISSN 1364-5528
R&D Projects: GA ČR GAP205/12/0466
Institutional support: RVO:68081707
Keywords : carbonic-anhydrase * hydrogen evolution * mercury-electrodes * water-molecules
Subject RIV: BO - Biophysics
Impact factor: 3.885, year: 2016

The transfer of protons or proton donor/acceptor abilities is an important phenomenon in many biomolecular systems. One example is the recently proposed peptidic proton-wires (H-wires), but the ability of these His-containing peptides to transfer protons has only been studied at the theoretical level so far. Here, for the first time the proton transfer ability of peptidic H-wires is examined experimentally in an adsorbed state using an approach based on a label-free electrocatalytic reaction. The experimental findings are complemented by theoretical calculations at the ab initio level in a vacuum and in an implicit solvent. Experimental and theoretical results indicated Ala(3)(His-Ala(2))(6) to be a high proton-affinity peptidic H-wire model. The methodology presented here could be used for the further investigation of the proton-exchange chemistry of other biologically or technologically important macromolecules.
Permanent Link: http://hdl.handle.net/11104/0269339