SGIP1 alters internalization and modulates signaling of activated cannabinoid receptor 1 in a biased manner

Hájková, Alena; Techlovská, Šárka; Dvořáková, Michaela; Chambers, Jayne Nicole; Kumpošt, Jiří; Hubálková, Pavla; Prezeau, L.; Blahoš, Jaroslav

doi:https://dx.doi.org/10.1016/j.neuropharm.2016.03.008

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SGIP1 alters internalization and modulates signaling of activated cannabinoid receptor 1 in a biased manner

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SYSNO ASEP	0471838
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	SGIP1 alters internalization and modulates signaling of activated cannabinoid receptor 1 in a biased manner
Author(s)	Hájková, Alena (UMG-J) Techlovská, Šárka (UMG-J) Dvořáková, Michaela (UMG-J) Chambers, Jayne Nicole (UMG-J) Kumpošt, Jiří (UMG-J) Hubálková, Pavla (UMG-J) Prezeau, L. (FR) Blahoš, Jaroslav (UMG-J)_{RID, ORCID}
Number of authors	8
Source Title	Neuropharmacology. - : Elsevier - ISSN 0028-3908 Roč. 107, léto (2016), s. 201-214
Number of pages	14 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Seven transmembrane receptors ; G-protein coupled receptors ; Cannabinoid receptor 1 ; Protein-protein interactions ; Bias signaling ; Receptor endocytosis
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GAP303/12/2408 GA ČR - Czech Science Foundation (CSF)
Institutional support	UMG-J - RVO:68378050
UT WOS	000378953400020
DOI	https://doi.org/10.1016/j.neuropharm.2016.03.008
Annotation	Many diseases of the nervous system are accompanied by alterations in synaptic functions. Synaptic plasticity mediated by the endogenous cannabinoid system involves the activation of the cannabinoid receptor 1 (CB1R). The principles of CB1R signaling must be understood in detail for its therapeutic exploration. We detected the Src homology 3-domain growth factor receptor-bound 2-like (endophilin) interacting protein 1 (SGIP1) as a novel CB1R partner. SGIP1 is functionally linked to clathrin-mediated endocytosis and its overexpression in animals leads to an energy regulation imbalance resulting in obesity. We report that SGIP1 prevents the endocytosis of activated CB1R and that it alters signaling via the CB1R in a biased manner. CB1R mediated G-protein activation is selectively influenced by SGIP1, arrestin associated signaling is changed profoundly, most likely as a consequence of the prevention of the receptor's internalization elicited by SGIP1. (C) 2016 The Authors. Published by Elsevier Ltd.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2017

Number of the records: 1