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Characterization of the Embryogenic Tissue of the Norway Spruce Including a Transition Layer between the Tissue and the Culture Medium by Magnetic Resonance Imaging
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SYSNO ASEP 0471601 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Characterization of the Embryogenic Tissue of the Norway Spruce Including a Transition Layer between the Tissue and the Culture Medium by Magnetic Resonance Imaging Author(s) Kořínek, Radim (UPT-D) RID, ORCID, SAI
Mikulka, J. (CZ)
Hřib, J. (CZ)
Hudec, Jiří (UPT-D) RID, ORCID, SAI
Havel, L. (CZ)
Bartušek, Karel (UPT-D) RID, ORCID, SAINumber of authors 6 Source Title Measurement Science Review. - : Sciendo - ISSN 1335-8871
Roč. 17, č. 1 (2017), s. 19-26Number of pages 8 s. Publication form Print - P Language eng - English Country SK - Slovakia Keywords Norway spruce ; ESEs ; mucilage ; transition layer ; MRI relaxometry Subject RIV BO - Biophysics OECD category Plant sciences, botany R&D Projects GA14-22777S GA ČR - Czech Science Foundation (CSF) GA13-09086S GA ČR - Czech Science Foundation (CSF) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000395820200003 EID SCOPUS 85014545354 DOI 10.1515/msr-2017-0003 Annotation The paper describes the visualization of the cells (ESEs) and mucilage (ECMSN) in an embryogenic tissue via magnetic resonance imaging (MRI) relaxometry measurement combined with the subsequent multi-parametric segmentation. The computed relaxometry maps T1 and T2 show a thin layer (transition layer) between the culture medium and the embryogenic tissue. The ESEs, mucilage, and transition layer differ in their relaxation times T1 and T2; thus, these times can be used to characterize the individual parts within the embryogenic tissue. The multi-parametric segmentation exploiting the T1 and T2 relaxation times as a classifier shows the distribution of the ESEs and mucilage within the embryogenic tissue. The discussed T1 and T2 indicators can be utilized to characterize both the growth-related changes in an embryogenic tissue and the effect of biotic/abiotic stresses, thus potentially becoming a distinctive indicator of the state of any examined embryogenic tissue.
Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2017
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