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2-Substituted dATP Derivatives as Building Blocks for Polymerase-Catalyzed Synthesis of DNA Modified in the Minor Groove
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SYSNO ASEP 0469642 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title 2-Substituted dATP Derivatives as Building Blocks for Polymerase-Catalyzed Synthesis of DNA Modified in the Minor Groove Author(s) Matyašovský, Ján (UOCHB-X) ORCID, RID
Perlíková, Pavla (UOCHB-X) RID, ORCID
Malnuit, Vincent (UOCHB-X)
Pohl, Radek (UOCHB-X) RID, ORCID
Hocek, Michal (UOCHB-X) RID, ORCIDSource Title Angewandte Chemie - International Edition. - : Wiley - ISSN 1433-7851
Roč. 55, č. 51 (2016), s. 15856-15859Number of pages 4 s. Language eng - English Country DE - Germany Keywords bioconjugation ; DNA modification ; DNA polymerase ; nucleotides ; fluorescent labelling Subject RIV CC - Organic Chemistry R&D Projects GA14-04289S GA ČR - Czech Science Foundation (CSF) Institutional support UOCHB-X - RVO:61388963 UT WOS 000390599400026 EID SCOPUS 85004154271 DOI 10.1002/anie.201609007 Annotation 2-Deoxyadenosine triphosphate (dATP) derivatives bearing diverse substituents (Cl, NH2, CH3, vinyl, ethynyl, and phenyl) at position 2 were prepared and tested as substrates for DNA polymerases. The 2-phenyl-dATP was not a substrate for DNA polymerases, but the dATPs bearing smaller substituents were good substrates in primer-extension experiments, producing DNA substituted in the minor groove. The vinyl-modified DNA was applied in thiol-ene addition and the ethynyl-modified DNA was applied in a CuAAC click reaction to form DNA labelled with fluorescent dyes in the minor groove. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2017 Electronic address http://onlinelibrary.wiley.com/doi/10.1002/anie.201609007/full
Number of the records: 1