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T7 polymerase-driven transcription is downregulated in metacyclic promastigotes and amastigotes of Leishmania mexicana
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SYSNO ASEP 0468978 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title T7 polymerase-driven transcription is downregulated in metacyclic promastigotes and amastigotes of Leishmania mexicana Author(s) Ishemgulova, A. (CZ)
Kraeva, N. (CZ)
Faktorová, Drahomíra (BC-A) RID, ORCID
Podešvová, L. (CZ)
Lukeš, Julius (BC-A) RID, ORCID
Yurchenko, V. (CZ)Number of authors 6 Article number 016 Source Title Folia Parasitologica. - : Biologické centrum AV ČR, v. v. i. - ISSN 0015-5683
Roč. 63, MAY 18 (2016)Number of pages 4 s. Publication form Online - E Language eng - English Country CZ - Czech Republic Keywords gene expression ; untranslated regions ; Tet-inducible system ; Leishmania mexicana Subject RIV EB - Genetics ; Molecular Biology Institutional support BC-A - RVO:60077344 UT WOS 000382606300001 EID SCOPUS 85017105620 DOI https://doi.org/10.14411/fp.2016.016 Annotation In our previous work we established a T7 polymerase-driven Tetracycline-inducible protein expression system in Leishmania mexicana (Biagi, 1953). We used this system to analyse gene expression profiles during development of L. mexicana in procyclic and metacyclic promastigotes and amastigotes. The transcription of the gene of interest and the T7 polymerase genes was significantly reduced upon cell differentiation. This regulation is not locus-specific. It depends on untranslated regions flanking open reading frames of the genes analysed. In this paper, we report that the previously established conventional inducible protein expression system may not be suitable for studies on differentiation of species of Leishmania Ross, 1903 and protein expression systems might have certain limitations. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2017
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