Heterologous Expression of Membrane Proteins: Methods and Protocols

0467873 - MBÚ 2017 RIV US eng M - Monography Chapter
Man, Petr - Forest, E.
Conformational dynamics and interactions of membrane proteins by hydrogen/deuterium mass spectrometry.
Heterologous Expression of Membrane Proteins: Methods and Protocols. 2. New York: Springer, 2016 - (Mus-Veteau, I.), s. 269-279. Methods in Molecular Biology, 1432. ISBN 978-1-4939-3635-9
R&D Projects: GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61388971
Keywords : Hydrogen/deuterium exchange * Mass spectrometry * Conformational change
OECD category: Biochemistry and molecular biology

Hydrogen/deuterium exchange associated with mass spectrometry has been recently used to characterize the dynamics and the interactions of membrane proteins. Here we describe experimental workflow enabling localization of the regions involved in conformational changes or interactions.
Permanent Link: http://hdl.handle.net/11104/0266196