Combined nano-biotechnology for in-situ remediation of mixed contamination of groundwater by hexavalent chromium and chlorinated solvents

Němeček, J.; Pokorný, P.; Lhotský, O.; Knytl, V.; Najmanová, P.; Steinová, J.; Černík, M.; Filipová, Alena; Filip, J.; Cajthaml, Tomáš

doi:https://dx.doi.org/10.1016/j.scitotenv.2016.01.019

Number of the records: 1

Combined nano-biotechnology for in-situ remediation of mixed contamination of groundwater by hexavalent chromium and chlorinated solvents

1.

SYSNO ASEP	0466606
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Combined nano-biotechnology for in-situ remediation of mixed contamination of groundwater by hexavalent chromium and chlorinated solvents
Author(s)	Němeček, J. (CZ) Pokorný, P. (CZ) Lhotský, O. (CZ) Knytl, V. (CZ) Najmanová, P. (CZ) Steinová, J. (CZ) Černík, M. (CZ) Filipová, Alena (MBU-M)_{RID, ORCID} Filip, J. (CZ) Cajthaml, Tomáš (MBU-M)_{RID, ORCID}
Source Title	Science of the Total Environment. - : Elsevier - ISSN 0048-9697 Roč. 563, SEP 1 (2016), s. 822-834
Number of pages	13 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Chlorinated solvents ; Hexavalent chromium ; NZVI
Subject RIV	EE - Microbiology, Virology
R&D Projects	TE01020218 GA TA ČR - Technology Agency of the Czech Republic (TA ČR)
Institutional support	MBU-M - RVO:61388971
UT WOS	000377792800085
EID SCOPUS	84956626643
DOI	10.1016/j.scitotenv.2016.01.019
Annotation	The present report describes a 13 month pilot remediation study that consists of a combination of Cr(VI) (4.4 to 57 mg/l) geofixation and dechlorination of chlorinated ethenes (400 to 6526 mu g/l), achieved by the sequential use of nanoscale zerovalent iron (nZVI) particles and in situ biotic reduction supported by whey injection. The remediation process was monitored using numerous techniques, including physical-chemical analyses and molecular biology approaches which enabled both the characterization of the mechanisms involved in pollutant transformation and the description of the overall background processes of the treatment. The results revealed that nZVI was efficient toward Cr(VI) by itself and completely removed it from the groundwater (LOQ 0.05 mg/l) and the subsequent application of whey resulted in a high removal of chlorinated ethenes (97 to 99%). The persistence of the reducing conditions, even after the depletion of the organic substrates, indicated a complementarity between nZVI and the whey phases in the combined technology as the subsequent application of whey phase partially assisted the microbial regeneration of the spent nZVI by promoting its reduction into Fe(II), which further supported remediation conditions at the site. Illumina sequencing and the detection of functional vcrA and bvcA genes documented a development in the reducing microbes (iron-reducing, sulfate-reducing and chlororespiring bacteria) that benefited under the conditions of the site and that was probably responsible for the high dechlorination and/or Cr(VI) reduction. The results of this study demonstrate the feasibility and high efficiency of the combined nano-biotechnological approach of nZVI and whey application in-situ for the removal of Cr(VI) and chlorinated ethenes from the groundwater of the contaminated si
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2017

Number of the records: 1