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Urinary metabolomic profiling in mice with diet-induced obesity and type 2 diabetes mellitus after treatment with metformin, vildagliptin and their combination
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SYSNO ASEP 0465861 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Urinary metabolomic profiling in mice with diet-induced obesity and type 2 diabetes mellitus after treatment with metformin, vildagliptin and their combination Author(s) Pelantová, Helena (MBU-M) ORCID, RID
Bugáňová, Martina (MBU-M)
Holubová, Martina (UOCHB-X) RID, ORCID
Šedivá, B. (CZ)
Zemenová, Jana (UOCHB-X)
Sýkora, D. (CZ)
Kaválková, P. (CZ)
Haluzík, M. (CZ)
Železná, Blanka (UOCHB-X) RID, ORCID
Maletínská, Lenka (UOCHB-X) RID, ORCID
Kuneš, Jaroslav (UOCHB-X) ORCID, RID
Kuzma, Marek (MBU-M) ORCID, RIDSource Title Molecular and Cellular Endocrinology. - : Elsevier - ISSN 0303-7207
Roč. 431, C (2016), s. 88-100Number of pages 13 s. Language eng - English Country IE - Ireland Keywords NMR metabolomics ; Mouse ; Obesity Subject RIV EE - Microbiology, Virology Subject RIV - cooperation Institute of Organic Chemistry and Biochemistry - Endocrinology, Diabetology, Metabolism, Nutrition R&D Projects GA13-14105S GA ČR - Czech Science Foundation (CSF) LO1509 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 ; UOCHB-X - RVO:61388963 UT WOS 000379276300009 EID SCOPUS 84971222599 DOI https://doi.org/10.1016/j.mce.2016.05.003 Annotation Metformin, vildagliptin and their combination are widely used for the treatment of diabetes, but little is known about the metabolic responses to these treatments. In the present study, NMR-based metabolomics was applied to detect changes in the urinary metabolomic profile of a mouse model of diet induced obesity in response to these treatments. Additionally, standard biochemical parameters and the expression of enzymes involved in glucose and fat metabolism were monitored. Significant correlations were observed between several metabolites (e.g., N-carbamoyl-beta-alanine, N1-methyl-4-pyridone-3-carboxamide, N1-methyl-2-pyridone-5-carboxamide, glucose, 3-indoxyl sulfate, dimethylglycine and several acylglycines) and the area under the curve of glucose concentrations during the oral glucose tolerance test. The present study is the first to present N-carbamoyl-beta-alanine as a potential marker of type 2 diabetes mellitus and consequently to demonstrate the efficacies of the applied antidiabetic interventions. Moreover, the elevated acetate level observed after vildagliptin administration might reflect increased fatty acid oxidation Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
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