Number of the records: 1
Plant Cytogenetics
- 1.
SYSNO ASEP 0464631 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Construction of BAC libraries from flow-sorted chromosomes Author(s) Šafář, Jan (UEB-Q) RID, ORCID
Šimková, Hana (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCIDSource Title Plant Cytogenetics, 1429. - New York : Humana Press, 2016 / Kianian S.F. ; Kianian P.M.A. - ISSN 1064-3745 - ISBN 978-1-4939-3620-5 Pages s. 135-149 Number of pages 15 s. Number of copy 3400 Number of pages 198 Publication form Print - P Language eng - English Country US - United States Keywords BAC library ; BAC vector ; Chromosomes Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000399777900013 EID SCOPUS 84990188418 DOI 10.1007/978-1-4939-3622-9_11 Annotation Cloned DNA libraries in bacterial artificial chromosome (BAC) are the most widely used form of large-insert DNA libraries. BAC libraries are typically represented by ordered clones derived from genomic DNA of a particular organism. In the case of large eukaryotic genomes, whole-genome libraries consist of a hundred thousand to a million clones, which make their handling and screening a daunting task. The labor and cost of working with whole-genome libraries can be greatly reduced by constructing a library derived from a smaller part of the genome. Here we describe construction of BAC libraries from mitotic chromosomes purified by flow cytometric sorting. Chromosome-specific BAC libraries facilitate positional gene cloning, physical mapping, and sequencing in complex plant genomes. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
Number of the records: 1