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Physiological role of FGF signaling in growth and remodeling of developing cardiovascular system
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SYSNO ASEP 0461572 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Physiological role of FGF signaling in growth and remodeling of developing cardiovascular system Author(s) Krejčí, Eliška (FGU-C) RID
Peševski, Živorad (FGU-C)
Naňka, O. (CZ)
Sedmera, David (FGU-C) RID, ORCID, SAISource Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 65, č. 3 (2016), s. 425-435Number of pages 11 s. Language eng - English Country CZ - Czech Republic Keywords chick embryo ; vasculogenesis ; FGF2 ; FGFR1 ; myocyte proliferation ; conotruncal banding ; SU5402 Subject RIV FA - Cardiovascular Diseases incl. Cardiotharic Surgery R&D Projects GAP302/11/1308 GA ČR - Czech Science Foundation (CSF) GA16-02972S GA ČR - Czech Science Foundation (CSF) Institutional support FGU-C - RVO:67985823 UT WOS 000382115700006 EID SCOPUS 84979771512 Annotation Fibroblast growth factor (FGF) signaling plays an important role during embryonic induction and patterning, as well as in modulating proliferative and hypertrophic growth in fetal and adult organs. Hemodynamically induced stretching is a powerful physiological stimulus for embryonic myocyte proliferation. The aim of this study was to assess the effect of FGF2 signaling on growth and vascularization of chick embryonic ventricular wall and its involvement in transmission of mechanical stretchinduced signaling to myocyte growth in vivo. Myocyte proliferation was significantly higher at the 48 h sampling interval in pressure-overloaded hearts. Neither Western blotting, nor immunohistochemistry performed on serial paraffin sections revealed any changes in the amount of myocardial FGF2 at that time point. ELISA showed a significant increase of FGF2 in the serum. Increased amount of FGF2 mRNA in the heart was confirmed by real time PCR. Blocking of FGF signaling by SU5402 led to decreased myocyte proliferation, hemorrhages in the areas of developing vasculature in epicardium and digit tips. FGF2 synthesis is increased in embryonic ventricular cardiomyocytes in response to increased stretch due to pressure overload. Inhibition of FGF signaling impacts also vasculogenesis, pointing to partial functional redundancy in paracrine control of cell proliferation in the developing heart. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2017
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