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Nanospray interfacing for capillary electrophoresis-mass spectrometry
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SYSNO ASEP 0459940 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Nanospray interfacing for capillary electrophoresis-mass spectrometry Author(s) Foret, František (UIACH-O) RID, ORCID
Křenková, Jana (UIACH-O) RID, ORCID
Týčová, Anna (UIACH-O) ORCID
Klepárník, Karel (UIACH-O) RID, ORCIDNumber of authors 4 Source Title Abstract book 40th ISCC and 13th GC×GC Symposium. - Messina : Chromaleont S.r.L, 2016 / Mondello L. ; Dugo P. - ISBN 978-88-941816-0-9
S. 117-117Number of pages 1 s. Publication form Medium - C Action ISCC /40./ and GC×GC Symposium /13./ Event date 29.05.2016-03.06.2016 VEvent location Riva del Garda Country IT - Italy Event type WRD Language eng - English Country IT - Italy Keywords CE/MS ; microfabricated liquid junction/sprayer module ; electrospray ionization ; separation capillaries Subject RIV CB - Analytical Chemistry, Separation Institutional support UIACH-O - RVO:68081715 Annotation Capillary electrophoresis coupled to mass spectrometry (CE/MS) is gaining its space among the most powerful tools in modern (bio)analytical laboratory. The most challenging instrumental aspect in CE/MS is striking the balance between the stability and reproducibility of the signal and sensitivity of the analysis. Several interface designs have been developed in the past decade addressing the variety of instrumental aspects and ease of operation. Most of the interfaces can be categorized either into the sheath flow arrangement (considered to be a de facto standard), or a sheathless arrangement, often expected to provide the ultimate sensitivity. In this work we have explored two, application dependent, approaches. A hybrid design, utilizing a microfabricated liquid junction/sprayer module, allowed coupling to a commercial, fully automated, CE analyser equipped with standard (50-75 um ID) separation capillaries. In the second, “interface-free” approach, the CE/MS analysis was performed in a narrow bore (um ID) separation capillary forming one entity with the electrospray tip. The high voltage, applied at the injection end of the capillary, served for both the separation and electrospray ionization. Optimum conditions for the separation and electrospray ionization were achieved with voltage programming. The performance of both CE/MS systems was tested for separations of metabolites, peptides and glycoproteins and compared to other designs with respect to the separation efficiency, sensitivity and sample consumption. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2017
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