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DNA damage response during mouse oocyte maturation
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SYSNO ASEP 0458917 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title DNA damage response during mouse oocyte maturation Author(s) Mayer, Alexandra (UZFG-Y) RID
Baran, Vladimír (UZFG-Y)
Sakakibara, Y. (JP)
Brzáková, Adéla (UZFG-Y)
Ferencová, Ivana (UZFG-Y) ORCID
Motlík, Jan (UZFG-Y) RID, ORCID
Kitajima, T. (JP)
Schultz, R. M. (US)
Šolc, Petr (UZFG-Y) RID, ORCIDSource Title Cell Cycle. - : Taylor & Francis - ISSN 1538-4101
Roč. 15, č. 4 (2016), s. 546-558Number of pages 13 s. Publication form Print - P Language eng - English Country US - United States Keywords double strand DNA breaks ; DNA damage ; MRE11 ; meiotic maturation ; mouse oocytes Subject RIV EB - Genetics ; Molecular Biology R&D Projects LH12057 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/03.0124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UZFG-Y - RVO:67985904 UT WOS 000372110000014 EID SCOPUS 84961285243 DOI https://doi.org/10.1080/15384101.2015.1128592 Annotation Because low levels of DNA double strand breaks (DSBs) appear not to activate the ATM-mediated prophase I checkpoint in full-grown oocytes, there may exist mechanisms to protect chromosome integrity during meiotic maturation. Using live imaging we demonstrate that low levels of DSBs induced by the radiomimetic drug Neocarzinostatin (NCS) increase the incidence of chromosome fragments and lagging chromosomes but do not lead to APC/C activation and anaphase onset delay. The number of DSBs, represented by gamma H2AX foci, significantly decreases between prophase I and metaphase II in both control and NCS-treated oocytes. Transient treatment with NCS increases >2-fold the number of DSBs in prophase I oocytes, but less than 30% of these oocytes enter anaphase with segregation errors. MRE11, but not ATM, is essential to detect DSBs in prophase I and is involved in H2AX phosphorylation during metaphase I. Inhibiting MRE11 by mirin during meiotic maturation results in anaphase bridges and also increases the number of gamma H2AX foci in metaphase II. Compromised DNA integrity in mirin-treated oocytes indicates a role for MRE11 in chromosome integrity during meiotic maturation. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2017
Number of the records: 1