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Voltammetric Behavior of 4-Aminophthalimide Label using Hanging Mercury Drop Electrode
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SYSNO ASEP 0456524 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Voltammetric Behavior of 4-Aminophthalimide Label using Hanging Mercury Drop Electrode Author(s) Ferenčíková, Z. (CZ)
Daňhel, Aleš (BFU-R) RID, ORCID
Riedl, Jan (UOCHB-X)
Hocek, Michal (UOCHB-X) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 5 Source Title Sborník přednášek mezinárodní odborné konference XXXIV. Moderní Elektrochemické Metody. - Ústí nad Labem : Best Servis, 2014 / Navrátil Tomáš ; Fojta M. ; Pecková K. - ISBN 978-80-905221-2-1 Pages s. 36-39 Number of pages 4 s. Publication form Print - P Action Moderní Elektrochemické Metody /34./ Event date 19.05.2014 - 23.05.2014 VEvent location Jetřichovice Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords labeled nucleotide ; 4-aminophthalimide ; primer extension ; DNA ; hanging mercury drop electrode ; voltammetry Subject RIV CG - Electrochemistry R&D Projects GBP206/12/G151 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 ; UOCHB-X - RVO:61388963 UT WOS 000353446900007 Annotation 4-aminophthalimide (API), covalently attached to nucleotide (dCTP or dATP) has recently beendesigned as a fluorescent label for the detection of DNA-protein interaction. However API is electrochemically reducible and therefore it is possible to use alternatively the API-modified nuclotides as DNA redox labels. The electrochemical behavior of labeled nucleotides and labeled DNA was studied using cyclic voltammetry and transfer stripping cyclic voltammetry at hanging mercury drop electrode. Observed CVs showed irreversible reduction signals of 4-aminophthalimide label without production of consequently oxidizable/reducible species. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2016
Number of the records: 1