Label-free electrochemical detection of singlet oxygen protein damage

Vargová, Veronika; Gimenez, R.E.; Černocká, Hana; Trujillo, D.C.; Tulli, F.; Zanini, V.I.P.; Paleček, Emil; Borsarelli, C.D.; Ostatná, Veronika

doi:https://dx.doi.org/10.1016/j.electacta.2015.11.104

Number of the records: 1

Label-free electrochemical detection of singlet oxygen protein damage

1.

SYSNO ASEP	0456295
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Label-free electrochemical detection of singlet oxygen protein damage
Author(s)	Vargová, Veronika (BFU-R) Gimenez, R.E. (AR) Černocká, Hana (BFU-R)_{RID, ORCID} Trujillo, D.C. (AR) Tulli, F. (AR) Zanini, V.I.P. (AR) Paleček, Emil (BFU-R)_{RID, ORCID} Borsarelli, C.D. (AR) Ostatná, Veronika (BFU-R)_{RID, ORCID}
Number of authors	10
Source Title	Electrochimica acta. - : Elsevier - ISSN 0013-4686 Roč. 187, JAN 2016 (2016), s. 662-669
Number of pages	8 s.
Publication form	Print - P
Language	eng - English
Country	GB - United Kingdom
Keywords	singlet oxygen protein damage ; surface-attached protein stability ; mercury and carbon electrodes
Subject RIV	BO - Biophysics
R&D Projects	GA13-00956S GA ČR - Czech Science Foundation (CSF)
Institutional support	BFU-R - RVO:68081707
UT WOS	000367235600077
DOI	10.1016/j.electacta.2015.11.104
Annotation	Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (O-1(2))-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2'-bipyridine)ruthenium(II) cation [Ru(bpy)(3)](2+) was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect oxidative changes in proteins. We believe that these methods will become important tools for detection of various protein damages. (C) 2015 Elsevier Ltd. All rights reserved.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2016

Number of the records: 1