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Voltammetric detection of phytochelatin transported across unmodified and protoplast modified model phospholipid membranes
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SYSNO ASEP 0453597 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Voltammetric detection of phytochelatin transported across unmodified and protoplast modified model phospholipid membranes Author(s) Navrátil, Tomáš (UFCH-W) RID, ORCID
Nováková, Kateřina (UFCH-W) RID
Josypčuk, Bohdan (UFCH-W) RID, SAI, ORCID
Sokolová, Romana (UFCH-W) RID, ORCID, SAI
Šestáková, Ivana (UFCH-W) RIDSource Title Monatshefte fur Chemie. - : Springer - ISSN 0026-9247
Roč. 147, č. 1 (2016), s. 165-171Number of pages 7 s. Language eng - English Country AT - Austria Keywords Barley protoplasts ; electrochemical impendance spectroscopy ; mercury electrode Subject RIV CG - Electrochemistry R&D Projects GAP208/12/1645 GA ČR - Czech Science Foundation (CSF) Institutional support UFCH-W - RVO:61388955 UT WOS 000367521400022 EID SCOPUS 84952802915 DOI 10.1007/s00706-015-1591-8 Annotation Possibility of phytochelatin PC2 transport was
examined using developed electrochemical cell with two
compartments separated by a phospholipid membrane
created in pores of polycarbonate support. At first cell
penetrating peptides (transportan 10 and mastoparan X)
were used to allow the transfer of phytochelatin PC2. The
results demonstrated that transportan 10 is several times
more efficient than mastoparan X for transferring of phytochelatin
across model membrane. Voltammetry with
hanging mercury drop electrode or with solid amalgam
electrodes was applied for detection of transported species.
For determination of PC2, hanging mercury drop electrode
and mercury meniscus-modified silver solid amalgam
electrode were suitable. Modified model phospholipid
membrane was prepared with parts of protoplasts from
barley roots or leaves, and transport of phytochelatin PC2
has been confirmed. Electrochemical impedance spectroscopy
was utilized to monitor formation, stability, and
qualitatively the transporting process across the supported
phospholipid membranes.Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2017
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