Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen

Lin, R.-H.; Lai, D.-H.; Zheng, L.-L.; Wu, J.; Lukeš, Julius; Hide, G.; Lun, Z.-R.

doi:https://dx.doi.org/10.1186/s13071-015-1281-8

Number of the records: 1

Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen

1.

SYSNO ASEP	0453402
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen
Author(s)	Lin, R.-H. (CN) Lai, D.-H. (CN) Zheng, L.-L. (CN) Wu, J. (CN) Lukeš, Julius (BC-A)_{RID, ORCID} Hide, G. (GB) Lun, Z.-R. (CN)
Source Title	Parasites & Vectors. - : BioMed Central - ISSN 1756-3305 Roč. 8, 30 December 2015 (2015), s. 665
Number of pages	11 s.
Publication form	Online - E
Language	eng - English
Country	GB - United Kingdom
Keywords	Trypanosoma lewisi ; Kinetoplast maxicircle ; Mitochondrial DNA ; RNA editing ; Palindrome
Subject RIV	EB - Genetics ; Molecular Biology
Institutional support	BC-A - RVO:60077344
UT WOS	000367326000001
DOI	10.1186/s13071-015-1281-8
Annotation	In this study, purified kinetoplast DNA from T. lewisi was sequenced using high-throughput sequencing in combination with sequencing of PCR amplicons. This allowed the assembly of the T. lewisi kinetoplast maxicircle DNA, which is a homologue of the mitochondrial genome in other eukaryotes. The assembly of 23,745 bp comprises the non-coding and coding regions. Comparative analysis of the maxicircle sequence of T. lewisi with Trypanosoma cruzi, Trypanosoma rangeli, Trypanosoma brucei and Leishmania tarentolae revealed that it shares 78 %, 77 %, 74 % and 66 % sequence identity with these parasites, respectively. The high GC content in at least 9 maxicircle genes of T. lewisi (ATPase6; NADH dehydrogenase subunits ND3, ND7, ND8 and ND9; G-rich regions GR3 and GR4; cytochrome oxidase subunit COIII and ribosomal protein RPS12) implies that their products may be extensively edited. A detailed analysis of the non-coding region revealed that it contains numerous repeat motifs and palindromes.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2016

Number of the records: 1